Human Phospho-FADD (S194) Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7047U Datasheet / COA / SDS

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FAB7047U-100UG

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Human Phospho-FADD (S194) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FADD when phosphorylated on Ser194.

Source

Monoclonal Mouse IgG2a Clone # 709736

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing the human FADD S194 site

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FADD

Human FADD (Fas-Associating protein with Death Domain), also known as MORT1, is a 25 kDa, 208 amino acid (aa) adaptor protein that mediates signaling of death domain-containing TNF receptor superfamily members and is thus a major regulator of apoptosis. FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity and autophagy. Phosphorylation on human FADD Ser194 (Ser191 in mouse) is found in T cells arrested at G2/M in the cell cycle; this effect is independent of death domain receptors. Human FADD shares 68% aa identity with mouse and rat FADD.

Long Name

Fas-associated Death Domain Protein

Entrez Gene IDs

8772 (Human)

Alternate Names

FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG3; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; MORT1; MORT1Fas-associating protein with death domain; protein FADD