Human Phospho-FADD (S194) Antibody

Catalog # Availability Size / Price Qty
MAB7047
MAB7047-SP
Detection of Human Phospho-FADD (S194) by Western Blot.
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Human Phospho-FADD (S194) Antibody Summary

Species Reactivity
Human
Specificity
Detects human FADD when phosphorylated on Ser194.
Source
Monoclonal Mouse IgG2A Clone # 709736
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human FADD S194 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-FADD (S194) antibody by Western Blot. View Larger

Detection of Human Phospho-FADD (S194) by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole or 4 mM hydroxyurea for 20 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FADD at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-FADD (S194) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-FADD (S194) in HeLa Human Cell Line. FADD phosphorylated at S194 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole using Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FADD

Human FADD (Fas-Associating protein with Death Domain), also known as MORT1, is a 25 kDa, 208 amino acid (aa) adaptor protein that mediates signaling of death domain-containing TNF receptor superfamily members and is thus a major regulator of apoptosis. FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity and autophagy. Phosphorylation on human FADD Ser194 (Ser191 in mouse) is found in T cells arrested at G2/M in the cell cycle; this effect is independent of death domain receptors. Human FADD shares 68% aa identity with mouse and rat FADD.

Long Name
Fas-associated Death Domain Protein
Entrez Gene IDs
8772 (Human)
Alternate Names
FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG3; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; MORT1; MORT1Fas-associating protein with death domain; protein FADD

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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