Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 709736
View all FADD Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human FADD S194 site

Specificity

Detects human FADD when phosphorylated on Ser194.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Phospho-FADD (S194) Antibody

Detection of Human Phospho-FADD (S194) antibody by Western Blot.

Detection of Human Phospho-FADD (S194) by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole or 4 mM hydroxyurea for 20 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FADD at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-FADD (S194) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Phospho-FADD (S194) in HeLa Human Cell Line.

FADD phosphorylated at S194 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole using Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-FADD (S194) Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole

Western Blot

1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line treated with nocodazole or hydroxyurea for 20 hours

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FADD

Human FADD (Fas-Associating protein with Death Domain), also known as MORT1, is a 25 kDa, 208 amino acid (aa) adaptor protein that mediates signaling of death domain-containing TNF receptor superfamily members and is thus a major regulator of apoptosis. FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity and autophagy. Phosphorylation on human FADD Ser194 (Ser191 in mouse) is found in T cells arrested at G2/M in the cell cycle; this effect is independent of death domain receptors. Human FADD shares 68% aa identity with mouse and rat FADD.

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Entrez Gene IDs

8772 (Human)

Gene Symbol

FADD

Additional FADD Products

Product Documents for Human Phospho-FADD (S194) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-FADD (S194) Antibody

For research use only

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail