Human Phospho-MEK1 (T292) Antibody PPS075: R&D Systems

Human Phospho-MEK1 (T292) Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Human ~45 kDa MEK1 phosphorylated at T292
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding human phospho-T292 of MEK1
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-MEK1 (T292) by Western Blot Western blot of recombinant Wild Type (WT) and mutant MEK1 with and without coexpression of MAP Kinase showing immunolabeling of the ~45 kDa MEK1 protein phosphorylated at T292.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: MEK1
Human MEK1 (MAPK/ERK kinase 1) is a 45 kDa member of the MAP kinase kinase subfamily of the Ser/Thr protein kinase family of enzymes. It is 393 amino acids (aa) in length and contains one ERK interaction (aa 1 - 32) and catalytic site (aa 68 - 361). There is one alternate splice form that shows a 26 aa deletion between aa 471 - 548. MEK1 is part of the MAP kinase pathway that is activated in response to growth factors and integrins. Two signal transduction pathways converge on MEK1. Mitogens activate Ras, which activates Raf, which activates MEK1. Integrins activate Rac, which activates PAK, which also activates MEK1. Raf activates MEK1 by phosphorylating S218 and S222 on MEK1. Alternatively, PAK indirectly activates MEK1 by phosphorylating MEK1 on S298, which promotes the Raf phosphorylation of S218 and S222. Once activated, MEK1 phosphorylates and activates ERK1/2. In a negative feedback loop, activated ERK can phosphorylate MEK1 on T292 and T386. This phosphorylation of T292 will interfere with PAK phosphorylation of MEK1 on S298. ERK-mediated phosphorylation of MEK1 at T386 may also interfere with PAK phosphorylation of MEK1.
  • References:
    1. Chong, H. et al. (2003) Cell. Signal. 15:463.
    2. Eblen, S.T. et al. (2004) Mol. Cell. Biol. 24:2308.
    3. Xu, B. et al. (1999) J. Biol. Chem. 274:34029.
    4. Frost, J.A. et al. (1997) EMBO J. 16:6426.
    5. Mansour, S.J. et al. (1994) J. Biochem. 116:304.
    6. Zheng, C-F. and K-L. Guan (1994) EMBO J. 13:1123.
  • Long Name:
    Mitogen-activated Protein Kinase Kinase 1
  • Entrez Gene IDs:
    5604 (Human); 26395 (Mouse)
  • Alternate Names:
    dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Related Research Areas
Isotype Controls
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Normal Rabbit IgG Control

Ctrl AB-105-C 42  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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