Human Phospho-PGC1 alpha (S571) Alexa Fluor® 488-conjugated Antibody

Catalog #: AF6650G Datasheet / COA / SDS

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AF6650G-100UG

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Human Phospho-PGC1 alpha (S571) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PGC1 alpha when phosphorylated at S571 in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing the human PGC1Î± S571 site.

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PGC1 alpha

PGC-1 alpha (PPAR-gamma coactivator 1; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG-1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC-1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Entrez Gene IDs

10891 (Human); 19017 (Mouse); 83516 (Rat)

Alternate Names

LEM6; Ligand effect modulator 6; ligand effect modulator-6; L-PGC-1alpha; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1; PGC-1(alpha); PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PGC-1v; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPARgamma coactivator 1alpha; PPAR-gamma coactivator 1-alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha