Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DPLG70
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Human PLA2G7/PAF-AH/Lp-PLA2 ELISA Standard Curve
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Citations (12)
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Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.284 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PLA2G7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human PLA2G7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure PLA2G7 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human PLA2G7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PLA2G7 showed linear curves that were parallel to the curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PLA2G7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.69 3.52 11.7 2.35 4.34 13
Standard Deviation 0.115 0.192 0.272 0.226 0.3 0.676
CV% 6.8 5.5 2.3 9.6 6.9 5.2

Recovery

The recovery of PLA2G7 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 85-105
EDTA Plasma (n=4) 101 91-112
Heparin Plasma (n=4) 102 92-106
Serum (n=4) 106 91-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PLA2G7 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PLA2G7/PAF-AH/Lp-PLA2 ELISA Linearity

Data Examples

Human PLA2G7/PAF-AH/Lp-PLA2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PLA2G7/PAF-AH/Lp-PLA2

Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.

Long Name:
Phospholipase A2 Group VII
Entrez Gene IDs:
7941 (Human); 27226 (Mouse); 301265 (Rat)
Alternate Names:
2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LpPLA2; Lp-PLA2; PAF acetylhydrolase; PAF-AH; PAFAHPAF 2-acylhydrolase; phospholipase A2, group VII (platelet-activating factor acetylhydrolase; PLA2G7; plasma); platelet-activating factor acetylhydrolase
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human PLA2G7/PAF-AH/Lp-PLA2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Rationale and design of the Mechanistic Substudy of the Randomized Trial to Prevent Vascular Events in HIV (REPRIEVE): Effects of pitavastatin on coronary artery disease and inflammatory biomarkers
    Authors: U Hoffmann, MT Lu, D Olalere, EC Adami, MT Osborne, A Ivanov, JS Aluru, S Lee, N Arifovic, ET Overton, CJ Fichtenbau, JA Aberg, B Alston-Smi, KL Klingman, M Waclawiw, TH Burdo, KC Williams, MV Zanni, P Desvigne-N, K Cooper-Arn, KV Fitch, H Ribaudo, PS Douglas, SK Grinspoon,
    Am. Heart J., 2019;212(0):1-12.
    Species: Human
    Sample Types: Plasma
  2. Treatment Effects of Jinlingzi Powder and Its Extractive Components on Gastric Ulcer Induced by Acetic Acid in Rats
    Authors: X Zhao, J Li, Y Meng, M Cao, J Wang
    Evid Based Complement Alternat Med, 2019;2019(0):7365841.
    Species: Rat
    Sample Types: Plasma
  3. Myeloperoxidase level and inflammatory markers and lipid and lipoprotein parameters in stable coronary artery disease
    Authors: E Kimak, B Zi?ba, D Duma, J Solski
    Lipids Health Dis, 2018;17(1):71.
    Species: Human
    Sample Types: Serum
  4. Change of Inflammatory Factors in Patients with Acute Coronary Syndrome
    Authors: CY Ma, ZY Xu, SP Wang, HY Peng, F Liu, JH Liu, FX Ren
    Chin. Med. J., 2018;131(12):1444-1449.
    Species: Human
    Sample Types: Serum
  5. Association between high-sensitivity C-reactive protein, lipoprotein-associated phospholipase A2 and carotid atherosclerosis: A cross-sectional study
    Authors: H Liu, Y Yao, Y Wang, L Ji, K Zhu, H Hu, J Chen, J Yang, Q Cui, B Geng, Q Liu, D Li, Y Zhou
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Exploration of serum sensitive biomarkers of fatty liver in dairy cows
    Authors: Y Shen, L Chen, W Yang, Z Wang
    Sci Rep, 2018;8(1):13574.
    Species: Bovine
    Sample Types: Serum
  7. Differential expression of Lp-PLA2 in obesity and type 2 diabetes and the influence of lipids
    Authors: L Jackisch, W Kumsaiyai, JD Moore, N Al-Daghri, I Kyrou, TM Barber, H Randeva, S Kumar, G Tripathi, PG McTernan
    Diabetologia, 2018;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  8. A Preliminary Study for Evaluating the Dose-Dependent Effect of d-Allulose for Fat Mass Reduction in Adult Humans: A Randomized, Double-Blind, Placebo-Controlled Trial
    Authors: Y Han, EY Kwon, MK Yu, SJ Lee, HJ Kim, SB Kim, YH Kim, MS Choi
    Nutrients, 2018;10(2):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  9. Measurement of Lipoprotein-Associated Phospholipase A2 by Use of 3 Different Methods: Exploration of Discordance between ELISA and Activity Assays
    Authors: C Topbas, A Swick, M Razavi, NL Anderson, TW Pearson, C Bystrom
    Clin. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  10. A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome
    Genome Biol, 2016;17(1):108.
    Species: Human
    Sample Types: Ascites Fluid
  11. Impact of cigarette smoking cessation on high-density lipoprotein functionality.
    Authors: Takata K, Imaizumi S, Kawachi E, Suematsu Y, Shimizu T, Abe S, Matsuo Y, Tsukahara H, Noda K, Yahiro E, Zhang B, Uehara Y, Miura S, Saku K
    Circ J, 2014;78(12):2955-62.
    Species: Human
    Sample Types: Plasma
  12. Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke.
    Authors: Lopez-Farre AJ, Zamorano-Leon JJ, Segura A, Mateos-Caceres PJ, Modrego J, Rodriguez-Sierra P, Calatrava L, Tamargo J, Macaya C
    J. Neurochem., 2012;121(2):314-25.
    Species: Human
    Sample Types: Plasma

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