Human Plexin B1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB3749X Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3749X-100UG

Save 15% on Select RUO Reagents. See Details.

All Plexin B1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin B1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Plexin B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Plexin C1, rhPlexin D1, recombinant mouse (rm) Plexin A1, rmPlexin A2, or rmPlexin A3 is observed.

Source

Monoclonal Mouse IgG1 Clone # 439512

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Plexin B1
Leu35-Val150
Accession # O43157

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with Neuropilins, MET, and EGF R2. Multiple splice variants are known. One shows a deletion of aa 689-871. A second shows a 53 aa substitution for aa 677‑2135. And a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Alternate Names

Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP