Detects human Plexin B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Plexin C1, rhPlexin D1, recombinant mouse (rm) Plexin A1, rmPlexin A2, or rmPlexin A3 is observed.
Monoclonal Mouse IgG1 Clone # 439512
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Plexin B1 Leu35-Val150 Accession # O43157
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Plexin B1 in Human Brain.
Plexin B1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB3749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin B1
Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with Neuropilins, MET, and EGF R2. Multiple splice variants are known. One shows a deletion of aa 689-871. A second shows a 53 aa substitution for aa 677‑2135. And a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.
Entrez Gene IDs:
5364 (Human); 235611 (Mouse); 316009 (Rat)
Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.