Human Plexin B1 Antibody

Catalog # Availability Size / Price Qty
MAB3749
MAB3749-SP
Human Plexin B1 Antibody in Immunohistochemistry
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Product Details
Citations (2)
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Human Plexin B1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Plexin C1, rhPlexin D1, recombinant mouse (rm) Plexin A1, rmPlexin A2, or rmPlexin A3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 439512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin B1
Leu35-Val150
Accession # O43157
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Plexin B1
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Plexin B1 in Human Brain. View Larger

Plexin B1 in Human Brain. Plexin B1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB3749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with Neuropilins, MET, and EGF R2. Multiple splice variants are known. One shows a deletion of aa 689-871. A second shows a 53 aa substitution for aa 677‑2135. And a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.

Entrez Gene IDs
5364 (Human); 235611 (Mouse); 316009 (Rat)
Alternate Names
Plexin B1; PLXN5; PLXNB1; SEP; Transmembrane Protein SEP

Product Datasheets

Citations for Human Plexin B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis.
    Authors: Worzfeld T, Swiercz JM, Looso M
    J. Clin. Invest., 2012;122(4):1296-305.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Grb2 mediates semaphorin-4D-dependent RhoA inactivation.
    Authors: Sun, Tianlian, Krishnan, Rameshku, Swiercz, Jakub M
    J Cell Sci, 2012;125(0):3557-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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