Human PlGF Antibody

Catalog # Availability Size / Price Qty
MAB264-SP
MAB264-100
MAB264-500
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human PlGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PlGF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF, rhPDGF‑AA, rhPDGF‑AB, or rhPDGF‑BB.
Source
Monoclonal Mouse IgG1 Clone # 37203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # P49763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human PlGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human PlGF Biotinylated Antibody (Catalog # BAF264)

Standard: Recombinant Human PlGF Protein (Catalog # 264-PGB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.

Long Name
Placenta Growth Factor
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names
D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760

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Citations for Human PlGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor
    Stem Cell Res Ther, 2016;7(1):173.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
    Authors: Park, Su-Jung, Kim, Ki-Jo, Kim, Wan-Uk, Cho, Chul-Soo
    J Immunol, 2014;192(7):3003-10.
    Species: Mouse
    Sample Types: Matrigel Plug
    Applications: Neutralization
  3. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Der p, IL-4, and TGF-beta cooperatively induce EGFR-dependent TARC expression in airway epithelium.
    Authors: Heijink IH, Marcel Kies P, van Oosterhout AJ, Postma DS, Kauffman HF, Vellenga E
    Am. J. Respir. Cell Mol. Biol., 2006;36(3):351-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PlGF Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human PlGF Antibody
By Anonymous on 02/17/2021
Application: ICC/IF Sample Tested: Endothelial colony-forming cells Species: Mouse

Cells were fixed in 4% paraformaldehyde for 20 min
at room temperature. Following PBS washes, cells were
permeabilised with 0.1% Triton X-100 for 10 min at RT
and then blocked in 1% bovine serum albumin for
2 h in PBS before overnight incubation in primary antibody
at 4 °C. After washing with PBS, cells were incubated
with appropriate secondary antibody for 1 h at RT and
imaged


Human PlGF Antibody
By Anonymous on 11/26/2020
Application: IHC Sample Tested: Placental tissue Species: Human

Human placenta were fixed in 4% paraformaldehyde. Tissue was embedded and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human PIGF Antigen was diluted in blocking buffer to 1:200 and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody.