Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all PLTP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1
Glu18-Val493 (Glu18Val)
Accession # P55058

Specificity

Detects human PLTP in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PLTP is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human PLTP Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PLTP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLTP

PLTP (phospholipid transfer protein) is a 75-81 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain; there is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa 110-162, and a second shows a four aa substitution for the N-terminal 35 amino acids. Over aa 18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively.

Long Name

Phospholipid Transfer Protein

Alternate Names

HDLCQ9, Lipid transfer protein II, phospholipid transfer protein

Entrez Gene IDs

5360 (Human); 18830 (Mouse); 296371 (Rat)

Gene Symbol

PLTP

UniProt

P55058 (Human)

Additional PLTP Products

Product Documents for Human PLTP Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLTP Biotinylated Antibody

For research use only

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