Human PLTP Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF5109
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Human PLTP Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLTP in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse PLTP is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1
Glu18-Val493 (Glu18Val)
Accession # P55058
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLTP

PLTP (phospholipid transfer protein) is a 75-81 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain; there is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa 110-162, and a second shows a four aa substitution for the N-terminal 35 amino acids. Over aa 18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively.

Long Name
Phospholipid Transfer Protein
Entrez Gene IDs
5360 (Human); 18830 (Mouse); 296371 (Rat)
Alternate Names
HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Staining Reagents

Avidin-Fluorescein

Supplemental Cell Selection Products

Supplemental ELISA Products

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