Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PLTP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PLTP isoform 1
Glu18-Val493 (Glu18Val)
Accession # P55058

Specificity

Detects human PLTP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PLTP is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PLTP Antibody

Detection of human PLTP antibody by Western Blot.

Detection of human PLTP by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human PLTP Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human PLTP Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PLTP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLTP at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLTP Antibody

Application
Recommended Usage

Knockout Validated

PLTP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PLTP knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLTP

PLTP (phospholipid transfer protein) is a 75-81 kDa, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476 amino acids (aa) in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain; there is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa 110-162, and a second shows a four aa substitution for the N-terminal 35 amino acids. Over aa 18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively.

Long Name

Phospholipid Transfer Protein

Alternate Names

HDLCQ9, Lipid transfer protein II, phospholipid transfer protein

Entrez Gene IDs

5360 (Human); 18830 (Mouse); 296371 (Rat)

Gene Symbol

PLTP

UniProt

P55058 (Human)

Additional PLTP Products

Product Documents for Human PLTP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLTP Antibody

For research use only

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