Human/Primate Chitinase 3-like 1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2599

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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Primate Chitinase 3-like 1 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate Chitinase 3-like 1 in ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Chitinase 3-like 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1 (R&D Systems, Catalog # 2599-CH)
Tyr22-Thr383
Accession # P36222
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Chitinase 3-like 1 (Catalog # 2599-CH)

Human/Primate Chitinase 3-like 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate Chitinase 3‑like 1 Antibody (Catalog # MAB25991)

Standard: Recombinant Human Chitinase 3-like 1 Protein, CF (Catalog # 2599-CH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5‑7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

References
  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
Entrez Gene IDs
1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)
Alternate Names
AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

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Citation for Human/Primate Chitinase 3-like 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer
    Authors: L Tiainen, M Hämäläinen, T Luukkaala, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, E Moilanen, PL Kellokumpu
    Clin. Breast Cancer, 2019;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection

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Reviews for Human/Primate Chitinase 3-like 1 Biotinylated Antibody

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Human/Primate Chitinase 3-like 1 Biotinylated Antibody
By Anonymous on 09/05/2018
Application: IP Sample Tested: Recombinant protein Species: Human

Western Blot of CHI3L1 using 2.5ug of RnD recombinant protein.