Chitinase 3-like 1/YKL-40 Antibody [Biotin]

R&D Systems | Catalog # BAF2599

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Chitinase 3-like 1/YKL-40 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1/YKL-40
Tyr22-Thr383
Accession # P36222

Specificity

Detects human and primate Chitinase 3-like 1/YKL-40 in ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Chitinase 3-like 1/YKL-40 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

Human Chitinase 3-like 1 ELISA Standard Curve

Human Chitinase 3-like 1 ELISA Standard Curve

Recombinant Human Chitinase 3‑like 1 (Catalog # 2599-CH) was serially diluted and captured by Rat Anti-Human/Primate Chitinase 3-like 1/YKL-40 Monoclonal Antibody (Catalog # MAB25991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Chitinase 3-like 1/YKL-40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Chitinase 3-like 1/YKL-40 (Catalog # 2599-CH)

Human/Primate Chitinase 3-like 1/YKL-40 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate Chitinase 3-like 1/YKL-40 Antibody (Catalog # MAB25991)
  • Standard: Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF (Catalog # 2599-CH)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF2599 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5‑7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

References

  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

UniProt

P36222

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

For research use only

Citations for Chitinase 3-like 1/YKL-40 Antibody [Biotin]

Customer Reviews for Chitinase 3-like 1/YKL-40 Antibody [Biotin] (1)

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  • Human/Primate Chitinase 3-like 1 Biotinylated Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/05/2018
    Western Blot of CHI3L1 using 2.5ug of RnD recombinant protein.
    Chitinase 3-like 1/YKL-40 Antibody [Biotin] BAF2599

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