Human Pro-MMP-1 Quantikine ELISA Kit

(27 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.095 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant human Pro-MMP-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0390.130.2410.0770.1760.312

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0220.0650.1770.0650.1530.27


The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-112
Heparin Plasma (n=5) 92 85-102
Serum (n=5) 97 87-112
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human Pro-MMP-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-1

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

    • Long Name
      Matrix Metalloproteinase 1
    • Entrez Gene IDs
      4312 (Human);
    • Alternate Names
      CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well.
    13.   For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.
      For Serum & Plasma Samples: Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    14. 50 µL Stop Solution
    15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 27
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    Sample Type
    1. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
      Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
      PLoS ONE, 2015;10(5):e0124865.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
      Authors: Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA
      Cytokine, 2012;59(1):184-90.
      Species: Human
      Sample Type: Subretinal fluid
    3. Anti-arthritic effects of magnolol in human interleukin 1β-stimulated fibroblast-like synoviocytes and in a rat arthritis model.
      Authors: Wang JH, Shih KS, Liou JP, Wu YW, Chang AS, Wang KL, Tsai CL, Yang CR
      PLoS ONE, 2012;7(2):e31368.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
      Authors: Li R, Ackerman WE, Mihai C
      PLoS ONE, 2012;7(6):e39766.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
      Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
      Eur. J. Haematol., 2010;84(3):239-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
      Authors: Hashizume M, Mihara M
      Biochem. Biophys. Res. Commun., 2010;403(2):184-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation.
      Authors: Roderfeld M, Rath T, Schulz R, Seeger W, Tschuschner A, Graf J, Roeb E
      J. Cyst. Fibros., 2009;8(5):338-47.
      Species: Human
      Sample Type: Serum
    8. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts.
      Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C
      Bone, 2009;44(6):1143-50.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia.
      Authors: Karapanagiotidis GT, Antonitsis P, Charokopos N, Foroulis CN, Anastasiadis K, Rouska E, Argiriadou H, Rammos K, Papakonstantinou C
      J Cardiothorac Surg, 2009;4(0):59.
      Species: Human
      Sample Type: Serum
    10. PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis.
      Authors: Hayer S, Pundt N, Peters MA, Wunrau C, Kuhnel I, Neugebauer K, Strietholt S, Zwerina J, Korb A, Penninger J, Joosten LA, Gay S, Ruckle T, Schett G, Pap T
      FASEB J., 2009;23(12):4288-98.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts.
      Authors: Wunrau C, Schnaeker EM, Freyth K, Pundt N, Wendholt D, Neugebauer K, Hansen U, Pap T, Dankbar B
      Arthritis Rheum., 2009;60(9):2606-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Prostaglandin E2 exerts catabolic effects in osteoarthritis cartilage: evidence for signaling via the EP4 receptor.
      Authors: Attur M, Al-Mussawir HE, Patel J, Kitay A, Dave M, Palmer G, Pillinger MH, Abramson SB
      J. Immunol., 2008;181(7):5082-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
      Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
      PLoS Med., 2008;5(4):e93.
      Species: Human
      Sample Type: Serum
    14. Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer.
      Authors: Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A
      Mol. Cancer Ther., 2008;7(9):2746-57.
      Species: Human
      Sample Type: ovarian ascites
    15. Inhibition of interleukin-1beta-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D2.
      Authors: Zayed N, Afif H, Chabane N, Mfuna-Endam L, Benderdour M, Martel-Pelletier J, Pelletier JP, Motiani RK, Trebak M, Duval N, Fahmi H
      Arthritis Rheum., 2008;58(11):3530-40.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Amidic alginate hydrogel for nucleus pulposus replacement.
      Authors: Leone G, Torricelli P, Chiumiento A, Facchini A, Barbucci R
      J Biomed Mater Res A, 2008;84(2):391-401.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
      Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
      Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
      Species: Human
      Sample Type: Tissue Homogenates
    18. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
      Authors: Naveau A, Reinald N, Fournier B
      Connect. Tissue Res., 2007;48(6):300-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
      Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
      FEBS Lett., 2007;581(9):1928-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. The potential of adiponectin in driving arthritis.
      Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U
      J. Immunol., 2006;176(7):4468-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures.
      Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ
      Arthritis Rheum., 2006;54(10):3152-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Humoral and cellular factors responsible for coronary collateral formation.
      Authors: Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M
      Am. J. Cardiol., 2006;98(9):1194-7.
      Species: Human
      Sample Type: Plasma
    23. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
      Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
      Eur. J. Immunol., 2006;36(5):1309-23.
    24. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1.
      Authors: Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T
      Biochem. Biophys. Res. Commun., 2005;338(4):1825-33.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells.
      Authors: Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC
      Invest. Ophthalmol. Vis. Sci., 2004;45(12):4302-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    26. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease.
      Authors: Ulrich D, Hrynyschyn K, Pallua N
      Plast. Reconstr. Surg., 2003;112(5):1279-86.
      Species: Human
      Sample Type: Serum
    27. Matrix metalloproteinases production in malignant pleural effusions after talc pleurodesis.
      Authors: D&amp;amp;apos;Agostino P
      Clin. Exp. Immunol., 2003;134(1):138-42.
      Species: Human
      Sample Type: Pleural Effusion
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    ELISA Controls
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    Quantikine Immunoassay Control Set 656 for Human Pro-MMP-1

    Ctrl QC125
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    Quantikine Wash Buffer 1

    ELISA WA126 5
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