Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 537331
View all Protein Disulfide Isomerase/P4HB Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protein Disulfide Isomerase/P4HB
Asp18-Lys505
Accession # P07237

Specificity

Conjugated Protein Disulfide Isomerase/P4HB antibodies are ideal for immunocytochemistry colocalization studies in the lumen of the endoplasmic reticulum. The unconjugated antibody detects human Protein Disulfide Isomerase/P4HB in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Protein Disulfide Isomerase/P4HB antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Protein Disulfide Isomerase/P4HB in HeLa Human Cell Line.

Protein Disulfide Isomerase/P4HB was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Protein Disulfide Isomerase/P4HB Biotinylated Monoclonal Antibody (Catalog # BAM4236) at 25 µg/mL overnight at 4 ° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein Disulfide Isomerase/P4HB

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family.  As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase.  This catalyses the hydroxylation of proline in collagen.  On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells.  Mature human P4HB is 491 amino acids (aa) in length.  It contains two TRX domains (aa 25-134 and 368-475) plus an ER retention sequence (aa 505-508).  There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18-505, human P4HB shares 94% aa identity with mouse P4HB. 

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Entrez Gene IDs

5034 (Human); 18453 (Mouse); 25506 (Rat)

Gene Symbol

P4HB

UniProt

P07237

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody

For research use only

Related Research Areas

Redox Enzymes

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