Detects human PSMA/FOLH1/NAALADase I in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) NAALADase-like 2 and no cross-reactivity with rhNAALADase-like 1, rhNAALADase-like 3, recombinant mouse (rm) PSMA/FOLH1/NAALADase I, or rmNAALAase-like 2 is observed.
Monoclonal Mouse IgG2A Clone # 460420
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I Lys44-Ala750 Accession # Q04609
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of PSMA/FOLH1/NAALADase I in LNCaP Human Cell Line by Flow Cytometry.
LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1/NAALADase I Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4234G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
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