Human PTP1B Antibody New

MAB1366 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13662-100
MAB13662-SP

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Human PTP1B Antibody in Immunohistochemistry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTP1B Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTP1B in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 208607

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PTP1B
Glu2-Thr435
Accession # P18031

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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PTP1B in Human Heart. PTP1B was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human PTP1B Monoclonal Antibody (Catalog # MAB13662) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP1B

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness.

References

Angers-Loustau, et al. (1999) Biochem. Cell Biol. 77:493.
Sarmiento, et al. (1998) J. Biol. Chem. 273:26368.
Bjorge, et al. (2000) J. Biol. Chem. 52:41439.
Balsamo, et al. (1996) J. Cell Biol. 134:801.
Elchebly, et al. (1999) Science 283:1544.

Long Name

Protein Tyrosine Phosphatase 1B

Entrez Gene IDs

5770 (Human); 19246 (Mouse)

Alternate Names

EC 3.1.3.48; protein tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP1B; PTP-1B; PTP1Bprotein tyrosine phosphatase, placental; PTPN1; tyrosine-protein phosphatase non-receptor type 1