Human PU.1/Spi-1 PE-conjugated Antibody

Name: Human PU.1/Spi-1 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC5870P
Price: 341 USD

Catalog #: IC5870P
1 Citation Datasheet / COA / SDS

Discontinued Product

IC5870P has been discontinued.

View all PU.1/Spi-1 products.

Detection of PU.1/Spi‑1 in Human PBMC lymphocytes by Flow Cytometry.

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All PU.1/Spi-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PU.1/Spi-1 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PU.1/Spi‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human SPI-B is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PU.1/Spi‑1
Met1-Lys169
Accession # NP_001074016

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of PU.1/Spi-1 antibody in Human PBMC lymphocytes antibody by Flow Cytometry.

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Detection of PU.1/Spi‑1 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Sheep Anti-Human PU.1/Spi-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5870P) and Mouse Anti-Human CD3e PerCP-conjugated Monoclonal Antibody (Catalog # FAB100C). Quadrant markers were set based on control antibody staining (Catalog # IC016P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of PU.1/Spi-1 antibody in Th2-differentiated Human PBMCs antibody by Flow Cytometry.

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Detection of PU.1/Spi‑1 in Th2-differentiated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 5 days, then treated overnight with PMA and Calcium Ionomycin to induce Th2 differentiation were stained with Sheep Anti-Human PU.1/Spi-1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5870P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC016P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: PU.1/Spi-1

PU.1 (Purine-rich Nucleic Acid Binding Protein 1), also known as 31 kDa Transforming Protein and SPI-1, is a member of the PU subfamily, ETS family of transcription factors. Although its predicted MW is 31 kDa, it appears to run anomalously high in SDS-PAGE at 40-45 kDa. PU.1 is a monomeric hematopoietic protein that regulates the differentiation of early myeloid and lymphoid progenitors. High PU.1 levels favor granulocyte and macrophage production, while low levels generate megakaryocytes, erythrocytes, T and B cells. Human PU.1 is 270 amino acids (aa) in length. It contains an N-terminal acidic/polyGln transactivation region (aa 34‑99), a non-destabilizing PEST sequence (aa 117-165) and a C-terminal Ets DNA-binding domain (aa 170-253). PU.1 is phosphorylated on Ser146, allowing it to bind to Pip. Over aa 1-169, human PU.1 shares 88% aa identity with mouse PU.1.

Long Name

Hematopoietic transcription factor PU.1

Entrez Gene IDs

6688 (Human); 20375 (Mouse); 366126 (Rat)

Alternate Names

hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SFPI131 kDa transforming protein; SPI-1 proto-oncogene; SPI1; Spi-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein; transcription factor PU.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human PU.1/Spi-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The Immunogenicity of Branded and Biosimilar Infliximab in Rheumatoid Arthritis According to Th9-Related Responses
Authors: R Talotta, A Berzi, A Doria, A Batticciot, MC Ditto, F Atzeni, P Sarzi-Putt, D Trabattoni
Int J Mol Sci, 2017-10-12;18(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry