Human IFN-gamma Antibody

  (10 citations)
(3 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat IFN-gamma and recombinant mouse IFN-gamma is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
    Met1-Gln144
    Accession # Q14609
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IFN-gamma (Catalog # 285-IF)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 5 ng/mL Recombinant Human IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.1‑0.3 µg/mL.
Immunocytochemistry
IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑285‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
  • Long Name:
    Interferon gamma
  • Entrez Gene IDs:
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
  • Alternate Names:
    IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
    Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W
    PLoS ONE, 2015;10(8):e0135999.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
    Authors: Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C
    Biochem. Biophys. Res. Commun., 2011;408(1):65-70.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  3. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011;7(4):e1002016.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010;116(12):2061-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  5. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2010;127(2):150-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
    Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
    Mucosal Immunol, 2008;1(5):412-20.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
    Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003;48(2):334-8.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  10. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 203  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 16  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4.3 (Based on 3 reviews)

Have you used Human IFN-gamma Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human IFN-gamma Antibody.

We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 3 applications: Immunohistochemistry, Immunocytochemistry/Immunofluorescence, ELISA.

Filter by Ratings
Excellent
(2)
Average
(1)
All Ratings
Filter by Applications
IHC
(1)
ICC/IF
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human IFN-gamma  Antibody AF-285-NA Array AF-285-NA
Enlarge
Excellent
 IHC Human Anonymous 04/13/2018
Show
Image Details
Immunohistochemistry Human IFN-gamma  Antibody AF-285-NA
Immunohistochemistry: Human IFN-gamma Antibody [AF-285-NA].

Summary

Application Immunohistochemistry
Sample Tested Adult brain
Species Human

Other Experimental Details

Other Experimental Details Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287Used at 10ug/ml.Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).
Immunocytochemistry/Immunofluorescence Human IFN-gamma  Antibody AF-285-NA Array AF-285-NA
Enlarge
Average
 ICC/IF Human Anonymous 10/01/2017
Show
Image Details
Immunocytochemistry/Immunofluorescence Human IFN-gamma  Antibody AF-285-NA
Immunocytochemistry/Immunofluorescence: Human IFN-gamma Antibody [AF-285-NA].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Kidney tissue (cortex)
Species Human
  Excellent
 ELISA Mouse Anonymous 06/29/2016
Show

Summary

Application ELISA
Sample Tested Activated mouse CD8 T cell
Species Mouse

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee