Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GOLGB1/Giantin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GOLGB1/Giantin
Ser1596-Glu1702
Accession # Q14789

Specificity

Detects human GOLGB1/Giantin in direct ELISAs. Detects human and rat GOLGB1/Giantin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Rat GOLGB1/Giantin Antibody

Detection of Human and Rat GOLGB1/Giantin antibody by Western Blot.

Detection of Human and Rat GOLGB1/Giantin by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK-OV-3 human ovarian adenocarcinoma cell line, ZR-75 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GOLGB1/Giantin antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

GOLGB1/Giantin in HeLa Human Cell Line.

GOLGB1/Giantin was detected in 2% paraformaldehyde immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat GOLGB1/Giantin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, ZR‑75 human breast cancer cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GOLGB1/Giantin

GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family.  GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton.  It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis.  GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome.  Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.

Long Name

Golgin B1

Alternate Names

GCP372, Giantin, GOLIM1, Macrogolgin

Entrez Gene IDs

2804 (Human); 224139 (Mouse); 192243 (Rat)

Gene Symbol

GOLGB1

UniProt

Q14789

Additional GOLGB1/Giantin Products

Product Documents for Human/Rat GOLGB1/Giantin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat GOLGB1/Giantin Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human/Rat GOLGB1/Giantin Antibody

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Protocols

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