Human/Rat GOLGB1/Giantin Antibody

Catalog # Availability Size / Price Qty
AF8159
AF8159-SP
Detection of Human and Rat GOLGB1/Giantin by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human/Rat GOLGB1/Giantin Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human GOLGB1/Giantin in direct ELISAs. Detects human and rat GOLGB1/Giantin in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GOLGB1/Giantin
Ser1596-Glu1702
Accession # Q14789
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Rat GOLGB1/Giantin by Western Blot. View Larger

Detection of Human and Rat GOLGB1/Giantin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, ZR‑75 human breast cancer cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GOLGB1/Giantin in HeLa Human Cell Line. View Larger

GOLGB1/Giantin in HeLa Human Cell Line. GOLGB1/Giantin was detected in 2% paraformaldehyde immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GOLGB1/Giantin

GOLGB1, also known as Giantin and Macrogolgin, is a 360 to 400 kDa member of the Golgin protein family.  GOLGB1 is an integral component of the Golgi membrane that associates with the cytoskeleton.  It may be involved in both forming intercisternal cross-bridges that mediate stacking in Golgi complexes, and in reorganizing the complexes after they fragment during mitosis.  GOLGB1 has also been identified as an autoantigen in chronic rheumatoid arthritis, and in the autoimmune disease Sjoegren syndrome.  Human GOLGB1 is 3259 amino acids (aa) in length, and over aa 1596-1702, shares 92% and 91% sequence identity with its mouse and rat orthologs, respectively.

Long Name
Golgin B1
Entrez Gene IDs
2804 (Human); 224139 (Mouse); 192243 (Rat)
Alternate Names
372 kDa Golgi complex-associated protein; GCP; GCP372; GCP372GIANTIN; Giantin; GOLGB1; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin; golgi integral membrane protein 1; golgin B1; golgin B1, golgi integral membrane protein; Golgin subfamily B member 1; GOLIM1; Macrogolgin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Rat GOLGB1/Giantin Antibody

There are currently no reviews for this product. Be the first to review Human/Rat GOLGB1/Giantin Antibody and earn rewards!

Have you used Human/Rat GOLGB1/Giantin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review