Human RBFOX3/NeuN Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB11690AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3202C
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Product Specifications

Specificity

Detects a synthetic peptide specific for human RBFOX3 around amino acid 25 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human RBFOX3/NeuN Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RBFOX3/NeuN

RBFOX3, also known as Neuronal nuclei (NeuN), is a 34kDa marker of post-mitotic neurons that is highly conserved  among different species.  The RBFOX family regulates alternative slicing and is encoded by three genes: RBFOX1, RBFOX2 and RBFOX3.  RBFOX3 mediates hippocampal circuitry, neurogenesis, and synaptogenesis. RBFOX3 plays a crucial role in normal synaptic function and is implicated in human neurological functions and mutations have been linked to neurodevelopmental delay, cognitive impairment, autistic features, and epilepsy.

References

  1. Wang HY, Hsieh PF, Huang DF, Chin PS, Chou CH, Tung CC, Chen SY, Lee LJ, Gau SS, Huang HS. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015 Dec 1;5:17383. doi: 10.1038/srep17383. PMID: 26619789; PMCID: PMC4664964. 
  2. Huang DF, Lee CY, Chou MY, Yang TY, Cao X, Hsiao YH, Wu RN, Lien CC, Huang YS, Huang HP, Gau SS, Huang HS. Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2203632119. doi: 10.1073/pnas.2203632119. Epub 2022 Aug 11. PMID: 35951651; PMCID: PMC9388145.

Long Name

RNA binding fox-1 homolog 3

Alternate Names

FOX-3, FOX3, HRNBP3, NEUN

Entrez Gene IDs

146713 (Human); 52897 (Mouse); 287847 (Rat)

Gene Symbol

RBFOX3

UniProt

Additional RBFOX3/NeuN Products

Product Documents for Human RBFOX3/NeuN Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RBFOX3/NeuN Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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