Human RBFOX3/NeuN Antibody

Detection of RBFOX3 in Human Brain Cortex via seqIF™ staining on COMET™ RBFOX3 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Rabbit Anti-Human RBFOX3, Monoclonal Antibody (Catalog # MAB11690) at 3ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the neuronal nuclei. Protocol available in COMET™ Panel Builder.

Detection of RBFOX3/NeuN in Human Cerebellum. RBFOX3/NeuN was detected in immersion fixed paraffin-embedded sections of cerebellum using Rabbit Anti-Human RBFOX3/NeuN Monoclonal Antibody (Catalog # MAB11690) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human RBFOX3/NeuN by Western Blot. Western Blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human RBFOX3/NeuN Monoclonal Antibody (Catalog # MAB11690) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific bands were detected for RBFOX3/NeuN at approximately 40 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of RBFOX3 in Frozen Mouse Hippocampus via seqIF™ staining on COMET™ RBFOX3 was detected in perfusion fixed-frozen sections of mouse Hippocampus using Rabbit Anti-Mouse RBFOX3, Monoclonal Antibody (Catalog# MAB11690) at 5ug/mL at 27° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5 minutes at room temperature followed by a 20 minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder.

Detection of RBFOX3 in Frozen Mouse Spinal Cord via seqIF™ staining on COMET™ RBFOX3 was detected in perfusion fixed-frozen sections of mouse Spinal Cord using Rabbit Anti-Mouse RBFOX3, Monoclonal Antibody (Catalog# MAB11690) at 5ug/mL at 27° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5minutes at room temperature followed by a 20 minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus. Protocol available in COMET™ Panel Builder.

Detection of RBFOX3 in Mouse Hippocampus. RBFOX3/NeuN was detected in immersion fixed paraffin-embedded sections of mouse hippocampus using Rabbit Anti-Human RBFOX3/NeuN Monoclonal Antibody (Catalog # MAB11690) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to specific staining localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.