Human RelA/NFkB p65 Antibody Summary
Accession # NP_068810
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of RelA/NF kappa B p65 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human RelA/NF kappa B p65 Monoclonal Antibody (Catalog # MAB50781, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RelA/NFkB p65
RelA p65 is a 65 kDa member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks amino acids (aa) 222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors.
Citation for Human RelA/NFkB p65 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
Authors: L Zhou, Y Huang, H Xie, X Mei
Mol Med Rep, 2017;0(0):.
Sample Types: Tissue Homogenates
Applications: Western Blot
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