Human Relaxin-1 Antibody

Catalog # Availability Size / Price Qty
MAB32572
MAB32572-SP
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Human Relaxin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects both the mature form and the proform of human Relaxin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Relaxin-2 or rhRelaxin-3 is observed.
Source
Monoclonal Rat IgG2A Clone # 361513
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Relaxin-1
Lys26-Cys185
Accession # NP_008842
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Relaxin‑1 (Catalog # 3257-RN) under non-reducing conditions only
Neutralization
Measured by its ability to neutralize Relaxin‑1-induced cAMP production in the THP‑1 human acute monocytic leukemia cell line. Parsell, D. A. et al. (1996) J. Biol. Chem. 271:27936. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human Relaxin‑1 (Catalog # 3257-RN).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Relaxin-1

Human Relaxin-1, also called H1 Relaxin or RLN1, is one of three human relaxins in the structurally related insulin/relaxin superfamily (1, 2). Relaxin-1 is thought to be the result of duplication of the Relaxin-2 gene in higher primates only. In species below higher primates, Relaxin-1 is the equivalent of human Relaxin-2. Relaxin-1 is found in some but not all tissues expressing Relaxin-2. It is prominent in the prostate, but also present in decidua, placenta, endometrium and at low levels in the myocardium (2, 3). As with other insulin/relaxin superfamily members, human Relaxin-1 is synthesized as a preprohormone (4). Processing of the 21 kDa preprorelaxin-1 includes removal of the signal sequence, formation of two disulfide bonds between A and B chains and removal of the intervening C-chain by a prohormone convertase. The resulting mature protein is an unglycosylated, 6 kDa dimer of disulfide-linked A and B chains. Human Relaxin-1 shares 76% amino acid (aa) identity with human Relaxin-2, and 43%, 50% and 43% aa identity with mouse, rat and canine Relaxin-1, respectively. An alternate splice form of unknown significance has a 47 aa substitution which does not have typical C-chain cleavage motifs (5). Relaxins confer activity by binding to leucine-rich G-protein coupled receptors LGR7 and LGR8 (2, 6). Prostatic relaxins are anti-apoptotic and contribute to development and maintenance of male fertility. It is not clear whether human Relaxins -1 and -2 have distinct functions. Both use the same receptor and have the same critical amino acids for folding and for receptor interaction. While receptor affinity is similar, activity is lower for Relaxin-1 as compared to Relaxin-2 (7). Progesterone increases expression of only Relaxin-2, while glucocorticoids increase expression of both (8).

References
  1. Hayes, E.S. (2004) Reprod. Biol. Endocrinol. 2:36.
  2. Sherwood, O.D. (2004) Endocr. Rev. 25:205.
  3. Wilkinson, T.N. et al. (2005) BMC Evol. Biol. 5:14.
  4. Hudson, P. et al. (1984) EMBO J. 3:2333.
  5. Gunnersen, J.M. et al. (1996) Mol. Cell. Endocrinol. 118:85.
  6. Hsu, S.Y. et al. (2002) Science 295:671.
  7. Schwabe, C. and E. E. Bullesbach (1994) FASEB J. 8:1152.
  8. Garibay-Tupas, J.L. et al. (2004) Mol. Cell. Endocrinol. 219:115.
Entrez Gene IDs
6013 (Human); 19773 (Mouse); 25616 (Rat)
Alternate Names
bA12D24.3.1; bA12D24.3.2; H1; preprorelaxin H1; prorelaxin H1; relaxin 1 (H1); relaxin 1; relaxin H1; Relaxin1; Relaxin-1; RLN1; RLXH1

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