Human Relaxin-2 Quantikine ELISA Kit DRL200: R&D Systems

Human Relaxin-2 Quantikine ELISA Kit

(5 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.57 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Relaxin-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC153, Quantikine Immunoassay Control Set 571 for Human Relaxin-2 - Please Inquire
Product Summary
The Quantikine Human Relaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Relaxin-2 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Relaxin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Relaxin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Relaxin-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020424242
Mean34.499.620640.8112220
Standard Deviation1.62.474.754.167.0212.1
CV%4.72.52.310.26.35.5

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020414141
Mean35.19619434.998.9197
Standard Deviation1.272.444.013.415.911.7
CV%3.62.52.19.865.9

Recovery

The recovery of Relaxin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 89-102
EDTA Plasma (n=4) 98 93-106
Heparin Plasma (n=4) 97 91-104
Serum (n=4) 101 92-107
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Relaxin-2 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Relaxin-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Relaxin-2
The Insulin/Relaxin superfamily includes Insulin, IGF-I, IGF-II, Relaxins, Relaxin-like Factor (RLF), Early Placenta Insulin-like Peptide 4 (INSL4), INSL5, and INSL6. Three human Relaxin genes (Relaxins-1 to -3) have been identified. Relaxin-2 is secreted and circulates in the blood, whereas Relaxin-1 is expressed in decidua, placenta, and prostate, and Relaxin-3 is expressed in the brain. Human Relaxin-2 is a peptide hormone that plays an important role in female reproduction.
    • Entrez Gene IDs
      6019 (Human);
    • Alternate Names
      bA12D24.1.1; bA12D24.1.2; H2; relaxin 2; Relaxin2; Relaxin-2; RLN2; RLXH2;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    5 Citations: Showing 1 - 5
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    Species
    Sample Type
    1. Serelaxin as a potential treatment for renal dysfunction in cirrhosis: Preclinical evaluation and results of a randomized phase 2 trial
      Authors: VK Snowdon, NJ Lachlan, AM Hoy, PW Hadoke, SI Semple, D Patel, W Mungall, TJ Kendall, A Thomson, RJ Lennen, MA Jansen, CM Moran, A Pellicoro, P Ramachandr, I Shaw, RL Aucott, T Severin, R Saini, J Pak, D Yates, N Dongre, JS Duffield, DJ Webb, JP Iredale, PC Hayes, JA Fallowfiel
      PLoS Med, 2017;14(2):e1002248.
      Species: Human
      Sample Type: Serum
    2. Serelaxin treatment reverses vascular dysfunction and left ventricular hypertrophy in a mouse model of Type 1 diabetes
      Authors: HH Ng, CH Leo, D Prakoso, C Qin, RH Ritchie, LJ Parry
      Sci Rep, 2017;7(0):39604.
      Species: Mouse
      Sample Type: Plasma
    3. Localization of relaxin receptors in arteries and veins, and region-specific increases in compliance and bradykinin-mediated relaxation after in vivo serelaxin treatment.
      Authors: Jelinic M, Leo C, Post Uiterweer E, Sandow S, Gooi J, Wlodek M, Conrad K, Parkington H, Tare M, Parry L
      FASEB J, 2014;28(1):275-87.
      Species: Rat
      Sample Type: Plasma
    4. Relaxin regulates hyaluronan synthesis and aquaporins in the cervix of late pregnant mice.
      Authors: Soh, Yu May, Tiwari, Anjana, Mahendroo, Mala, Conrad, Kirk P, Parry, Laura J
      Endocrinology, 2012;153(12):6054-64.
      Species: Human
      Sample Type: Complex Sample Type
    5. Serum relaxin levels are reduced in pregnant women with a history of recurrent miscarriage, and correlate with maternal uterine artery Doppler indices in first trimester.
      Authors: Anumba DO, El Gelany S, Elliott SL
      Eur. J. Obstet. Gynecol. Reprod. Biol., 2009;147(1):41-5.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 571 for Human Relaxin-2

    Ctrl QC153
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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