Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all S100A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A1
Met1-Ser94
Accession # P23297

Specificity

Detects human S100A1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human S100A1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human S100A1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A1

S100A1 (also S100 alpha) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Extracellularly, it exists as both a homodimer and heterodimer with S100B, S100A4 and S100P, may bind to RAGE, and blocks apoptosis. Human S100A1 is 94 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85) and a Ca-dependent protein interaction site (aa 89‑91). There may be one alternate start site 53 aa upstream of the standard start site. Full-length human S100A1 shares 93% and 98% aa identity with mouse and canine S100A1, respectively.

Long Name

S100 Calcium Binding Protein A1

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Entrez Gene IDs

6271 (Human); 20193 (Mouse); 295214 (Rat)

Gene Symbol

S100A1

UniProt

P23297

Additional S100A1 Products

Product Documents for Human S100A1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A1 Biotinylated Antibody

For research use only

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