Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all S100A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human S100A1
Met1-Ser94
Accession # P23297

Specificity

Detects human S100A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) S100B, rhS100P, and rhS100A2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human S100A1 Antibody

Detection of Human S100A1 antibody by Western Blot.

Detection of Human S100A1 by Western Blot.

Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human S100A1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart and brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A1

S100A1 (also S100 alpha) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Extracellularly, it exists as both a homodimer and heterodimer with S100B, S100A4 and S100P, may bind to RAGE, and blocks apoptosis. Human S100A1 is 94 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 50‑85) and a Ca-dependent protein interaction site (aa 89‑91). There may be one alternate start site 53 aa upstream of the standard start site. Full-length human S100A1 shares 93% and 98% aa identity with mouse and canine S100A1, respectively.

Long Name

S100 Calcium Binding Protein A1

Alternate Names

protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Entrez Gene IDs

6271 (Human); 20193 (Mouse); 295214 (Rat)

Gene Symbol

S100A1

UniProt

P23297

Additional S100A1 Products

Product Documents for Human S100A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S100A1 Antibody

For research use only

Citations for Human S100A1 Antibody

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