Human S100A13 DuoSet ELISA

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
    • Assay Type
      Solid Phase Sandwich ELISA
    • Format
      96-well strip plate
    • Sample Volume Required
      100 µL
    • Sufficient Materials
      For five 96-well plates*
    • Specificity
      Please see the product datasheet
    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
    Ancillary Reagent Kit Available
    DY008 , DuoSet ELISA Ancillary Reagent Kit 2 - Please Inquire
    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human S100A13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

    Product Features
    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
    • Development protocols are provided to guide further assay optimization
    • Assay can be customized to your specific needs
    • Economical alternative to complete kits
    Kit Content
    • Capture Antibody
    • Detection Antibody
    • Recombinant Standard
    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
    Other Reagents Required

    PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

    Wash Buffer: (Catalog # WA126), or equivalent

    Reagent Diluent*

    Blocking Buffer*

    Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

    Stop Solution: 2 N H2SO4 (Catalog # DY994)

    Microplates: R&D Systems (Catalog # DY990), or equivalent

    Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

    Product Datasheets

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Certificate of Analysis Lookup

    Certificate of Analysis Request Form

    To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Preparation and Storage
    • Stability & Storage
      Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    Background: S100A13

    S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca2+-binding proteins. It is widely expressed as a homodimer with two 98 amino acid (aa) long subunits. Human S100A13 shares 83%, 90%, 91%, 87%, 78% and 47% aa identity with mouse, rat, cow, dog, opossum, and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue rich sequence at the C-terminus, and two EF hand motifs that bind with Ca2+ differing affinities.

    Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation endproducts (RAGE). Despite lacking a signal sequence, S100A13 plays an important role in Cu2+-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation. Binding of copper is necessary for formation of a multiprotein complex between S100A13, FGF-1 and p40 synaptotagmin1 (syt1). Cu2+ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export. calcium influx may also play a similar role in FGF-1 release from neuronal cells. With FGF-1 and syt1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell. S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress induced export of FGF-1.

    • Long Name:
      S100 Calcium Binding Protein
    • Entrez Gene IDs:
      6284 (Human); 20196 (Mouse); 295213 (Rat)
    • Alternate Names:
      S100 calcium binding protein A13; S100 calcium-binding protein A13protein S100-A13; S100A13
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles
    Authors: CH Hulme, EL Wilson, MJ Peffers, S Roberts, DM Simpson, JB Richardson, P Gallacher, KT Wright
    Arthritis Res. Ther., 2017;19(1):150.
    Species: Human
    Sample Type: Synovial Fluid

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
DuoSet Ancillary Reagent Kits
Description Application Cat# Citations Images  

DuoSet ELISA Ancillary Reagent Kit 2

ELISA DY008 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack

ELISA DY999 65  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 2

ELISA DY995 18  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Stop Solution 2N Sulfuric Acid

ELISA DY994 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate Sealers

ELISA DY992  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-HRP

ELISA DY998 51  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Clear Polystyrene Microplates

ELISA DY990 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Additive 1

ELISA DY005 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Glo Substrate Reagent Pack

ELISA DY993 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate-coating Buffer

ELISA DY006 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 3 (5X)

ELISA DY004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Black Polystyrene Microplates, 25 Pack

ELISA DY991 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human S100A13 DuoSet ELISA?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee