Human S100A13 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human S100A13 ELISA Standard Curve
Preparation and Storage
S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca2+-binding proteins. It is widely expressed as a homodimer with two 98 amino acid (aa) long subunits. Human S100A13 shares 83%, 90%, 91%, 87%, 78% and 47% aa identity with mouse, rat, cow, dog, opossum, and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue rich sequence at the C-terminus, and two EF hand motifs that bind with Ca2+ differing affinities.
Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation endproducts (RAGE). Despite lacking a signal sequence, S100A13 plays an important role in Cu2+-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation. Binding of copper is necessary for formation of a multiprotein complex between S100A13, FGF-1 and p40 synaptotagmin1 (syt1). Cu2+ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export. calcium influx may also play a similar role in FGF-1 release from neuronal cells. With FGF-1 and syt1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell. S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress induced export of FGF-1.
Citation for Human S100A13 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles
Authors: CH Hulme, EL Wilson, MJ Peffers, S Roberts, DM Simpson, JB Richardson, P Gallacher, KT Wright
Arthritis Res. Ther., 2017;19(1):150.
Sample Types: Synovial Fluid
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