Detects human S100A16 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse S100A16 and less than 1% cross-reactivity with recombinant human (rh) S100A13 and rhS100B.
Polyclonal Sheep IgG
E. coli-derived recombinant human S100A16 Ser2-Ser103 Accession # Q96FQ6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
S100A16 in Human Brain. S100A16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human S100A16 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
S100A16 (also S100F) is a 12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. By Northern blot, S100A16 is found in multiple tissue types; in brain, it is specifically found in astrocytes. Intracellularly, S100A16 is both nuclear and cytoplasmic and apparently exists as a homodimer, trimer and tetramer.
S100 Calcium Binding Protein A16
Entrez Gene IDs:
140576 (Human); 67860 (Mouse); 361991 (Rat)
AAG13; Aging-associated gene 13 protein; aging-associated protein 13; DT1P1A7; MGC17528; protein S100-A16; Protein S100-F; S100 calcium binding protein A16; S100A16; S100F; S100FS100 calcium-binding protein A16
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