Human S100A16 Antibody Summary
Accession # Q96FQ6
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
S100A16 in Human Brain. S100A16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human S100A16 by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5180) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
S100A16 (also S100F) is a 12 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. By Northern blot, S100A16 is found in multiple tissue types; in brain, it is specifically found in astrocytes. Intracellularly, S100A16 is both nuclear and cytoplasmic and apparently exists as a homodimer, trimer and tetramer.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human S100A16 Antibody
There are currently no reviews for this product. Be the first to review Human S100A16 Antibody and earn rewards!
Have you used Human S100A16 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image