Human SART1 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8034T Datasheet / COA / SDS

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FAB8034T-100UG

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Human SART1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SART1 in ELISAs and Western Blots. Detects mouse SART1 in Western Blots.

Source

Monoclonal Mouse IgG1 Clone # 865709

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human SART1
Asn647-Lys800
Accession # O43290

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SART1

Hypoxia-associated factor (HAF), also known as SART1, is a widely expressed 125 kDa intracellular protein that promotes assembly of the U2-type pre-mRNA spliceosome. SART1 is also an E3 ubiquitin ligase that promotes the ubiquitination and degradation of HIF-1 alpha. In contrast, it enhances the transcriptional activity of HIF-2 alpha. SART1 is upregulated in many tumor types and enables tumor growth during chronic hypoxia. Within amino acids 647-800, human SART1 shares 100% aa sequence identity with mouse and rat SART1.

Long Name

Squamous cell carcinoma antigen recognized by T cells

Entrez Gene IDs

9092 (Human); 20227 (Mouse); 29678 (Rat)

Alternate Names

Allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia-associated factor; IgE Autoantigen; MGC2038; SART1; SART-1; SART1(259) protein; SART1(800) protein; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; SNU66 homolog; Snu66; squamous cell carcinoma antigen recognised by T cells; Squamous cell carcinoma antigen recognized by T cells 1; squamous cell carcinoma antigen recognized by T cells; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1