Human SCP3/SYCP3 Antibody

Detection of Mouse SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence SETDB1-dependent H3K9me3 is dispensable for the initiation of MSCI.a Schematic of the strategy for generating Setdb1-cKO mice. b Evaluation of the efficiency of SETDB1 deletion shown as the mean and individual values for 3 independent samples. c Chromosome spreads of early and mid pachytene spermatocytes, distinguished by the absence (early) or the presence (mid) of H1T, respectively (not shown), immunostained with antibodies raised against SYCP3 and H3K9me3. XY chromosomes are indicated with dashed squares and are magnified in the panels to the right. Putative regions of XY chromatin are indicated by dashed circles. d Chromosome spreads of mid pachytene spermatocytes immunostained with antibodies raised against SYCP3, gamma H2AX, and Pol II. e A population of pachytene spermatocytes with a normal gamma H2AX domain and normal MSCI, as defined by gamma H2AX and Pol II, respectively. The mean and individual values for 3 independent samples are shown. EP early pachytene, MP mid pachytene, X-PCH X-pericentromeric heterochromatin. Scale bars: 10 μm. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36443288), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunohistochemistry Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunohistochemistry Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence SETDB1-dependent H3K9me3 is dispensable for the initiation of MSCI.a Schematic of the strategy for generating Setdb1-cKO mice. b Evaluation of the efficiency of SETDB1 deletion shown as the mean and individual values for 3 independent samples. c Chromosome spreads of early and mid pachytene spermatocytes, distinguished by the absence (early) or the presence (mid) of H1T, respectively (not shown), immunostained with antibodies raised against SYCP3 and H3K9me3. XY chromosomes are indicated with dashed squares and are magnified in the panels to the right. Putative regions of XY chromatin are indicated by dashed circles. d Chromosome spreads of mid pachytene spermatocytes immunostained with antibodies raised against SYCP3, gamma H2AX, and Pol II. e A population of pachytene spermatocytes with a normal gamma H2AX domain and normal MSCI, as defined by gamma H2AX and Pol II, respectively. The mean and individual values for 3 independent samples are shown. EP early pachytene, MP mid pachytene, X-PCH X-pericentromeric heterochromatin. Scale bars: 10 μm. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36443288), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence SETDB1-dependent H3K9me3 is dispensable for the initiation of MSCI.a Schematic of the strategy for generating Setdb1-cKO mice. b Evaluation of the efficiency of SETDB1 deletion shown as the mean and individual values for 3 independent samples. c Chromosome spreads of early and mid pachytene spermatocytes, distinguished by the absence (early) or the presence (mid) of H1T, respectively (not shown), immunostained with antibodies raised against SYCP3 and H3K9me3. XY chromosomes are indicated with dashed squares and are magnified in the panels to the right. Putative regions of XY chromatin are indicated by dashed circles. d Chromosome spreads of mid pachytene spermatocytes immunostained with antibodies raised against SYCP3, gamma H2AX, and Pol II. e A population of pachytene spermatocytes with a normal gamma H2AX domain and normal MSCI, as defined by gamma H2AX and Pol II, respectively. The mean and individual values for 3 independent samples are shown. EP early pachytene, MP mid pachytene, X-PCH X-pericentromeric heterochromatin. Scale bars: 10 μm. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36443288), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunohistochemistry Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunohistochemistry Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence Expression of SYCP3 and gamma H2AX in the testis of the patient with SYCE1 CNVs and OA-affected patient as a positive control. A–C Immunofluorescence staining showed the expression of SYCP3 (Green) and gamma H2AX (Red) in the testis of the patient P6326 (A), P10377 (B), and the patient with OA as a positive control (C) Scale bars = 20 μm in A–D. And the arrowhead indicated the XY body in the spermatocytes in the testis of the patient with OA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35718780), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SCP3/SYCP3 by Immunocytochemistry/ Immunofluorescence SETDB1-dependent H3K9me3 is dispensable for the initiation of MSCI.a Schematic of the strategy for generating Setdb1-cKO mice. b Evaluation of the efficiency of SETDB1 deletion shown as the mean and individual values for 3 independent samples. c Chromosome spreads of early and mid pachytene spermatocytes, distinguished by the absence (early) or the presence (mid) of H1T, respectively (not shown), immunostained with antibodies raised against SYCP3 and H3K9me3. XY chromosomes are indicated with dashed squares and are magnified in the panels to the right. Putative regions of XY chromatin are indicated by dashed circles. d Chromosome spreads of mid pachytene spermatocytes immunostained with antibodies raised against SYCP3, gamma H2AX, and Pol II. e A population of pachytene spermatocytes with a normal gamma H2AX domain and normal MSCI, as defined by gamma H2AX and Pol II, respectively. The mean and individual values for 3 independent samples are shown. EP early pachytene, MP mid pachytene, X-PCH X-pericentromeric heterochromatin. Scale bars: 10 μm. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36443288), licensed under a CC-BY license. Not internally tested by R&D Systems.