Human Semaphorin 5A Antibody

Catalog # Availability Size / Price Qty
MAB5896
MAB5896-SP
Semaphorin 5A in Human Pancreatic Cancer Tissue.
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Human Semaphorin 5A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 5A in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 914419
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Semaphorin 5A
Glu23-Thr765
Accession # Q13591
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Semaphorin 5A in Human Pancreatic Cancer Tissue. View Larger

Semaphorin 5A in Human Pancreatic Cancer Tissue. Semaphorin 5A was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Semaphorin 5A Monoclonal Antibody (Catalog # MAB5896) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 5A

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1‑4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains
(1‑3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (4‑6). The human Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 22 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23‑765, which includes the sema domain and four of the seven TSP repeats, human Sema5A shares 93% aa identity with corresponding mouse, rat, and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (3, 11). Sema5A expression may be upregulated in metastatic cancer cells and downregulated in autism (12, 13).

References
  1. Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.
  2. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  3. Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.
  4. Oster, S.F. et al. (2003) Development 130:775.
  5. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  6. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
  7. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  8. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  9. Kantor, D.B. et al. (2004) Neuron 44:961.
  10. Hilario, J.D. et al. (2008) Dev. Biol. 326:190.
  11. Lin, L. et al. (2009) Trends Neurosci. 32:142.
  12. Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.
  13. Melin, M. et al. (2006) Neuropsychobiology 54:64.
Entrez Gene IDs
9037 (Human); 20356 (Mouse); 310207 (Rat)
Alternate Names
FLJ12815; sema domain, seven thrombospondin repeats (type 1 and type 1-like); Sema F; SEMA5A; SEMAF; Semaphorin 5A; semaphorin F; semaphorin-5A; Semaphorin-F; semF; transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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