Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.
Human Semaphorin 7A Biotinylated Antibody
R&D Systems | Catalog # BAF2068
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
His47-Ala648
Accession # O75326
Specificity
Detects human Semaphorin 7A in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Semaphorin 7A is observed and less than 1% cross-reactivity with recombinant human Semaphorin 3A, 6B, 6D and rmSema 3C, 3E, and 3F is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Semaphorin 7A Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7)
Sample: Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Semaphorin 7A
Alternate Names
CD108, H-Sema-L, Sema7A, SEMAL
Gene Symbol
SEMA7A
UniProt
Additional Semaphorin 7A Products
Product Documents for Human Semaphorin 7A Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Semaphorin 7A Biotinylated Antibody
For research use only
Related Research Areas
Citations for Human Semaphorin 7A Biotinylated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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