Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.
Human Semaphorin 7A Antibody [Biotin]
R&D Systems | Catalog # BAF2068
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Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
His47-Ala648
Accession # O75326
Specificity
Detects human Semaphorin 7A in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Semaphorin 7A is observed and less than 1% cross-reactivity with recombinant human Semaphorin 3A, 6B, 6D and rmSema 3C, 3E, and 3F is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Semaphorin 7A Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7)
Sample: Recombinant Human Semaphorin 7A Fc Chimera (Catalog # 2068-S7)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Semaphorin 7A
Alternate Names
CD108, H-Sema-L, Sema7A, SEMAL
Gene Symbol
SEMA7A
UniProt
Additional Semaphorin 7A Products
Product Documents for Human Semaphorin 7A Antibody [Biotin]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Semaphorin 7A Antibody [Biotin]
For research use only
Related Research Areas
Citations for Human Semaphorin 7A Antibody [Biotin]
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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