Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Semaphorin 7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326

Specificity

Detects human Semaphorin 7A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Semaphorin 7A is observed. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Semaphorin 7A Antibody

Detection of Semaphorin 7A in PBMC lymphocytes treated +/- 5 µg/mL PHA for 2 days by Flow Cytometry

PBMC lymphocytes treated with 5 µg/mL PHA for 2 days (A) vs naïve PBMC lymphocytes (B) were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse anti-Human CD3 APC-conjugated Monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Detection of Semaphorin 7A in HT1080 cells by Flow Cytometry

HT1080 cells were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Semaphorin 7A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human T cells treated with PHA (see details below); HT1080 human fibrosarcoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Semaphorin 7A

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 7A

Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.

Alternate Names

CD108, H-Sema-L, Sema7A, SEMAL

Entrez Gene IDs

8482 (Human); 20361 (Mouse)

Gene Symbol

SEMA7A

UniProt

O75326

Additional Semaphorin 7A Products

Product Documents for Human Semaphorin 7A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Semaphorin 7A Antibody

For research use only

Related Research Areas

Proteoglycans
Red Blood Cells (RBCs)

Citations for Human Semaphorin 7A Antibody

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