Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Serpin A8/Angiotensinogen Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A8
Asp34-Ala485
Accession # ABM85122

Specificity

Detects human Serpin A8 in Western blots. In this format, less than 1% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Serpin A8/Angiotensinogen Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin A8/Angiotensinogen

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human angiotensinogen is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.        

Alternate Names

AGT, Angiotensin II, Angiotensinogen, Aogen, PAT

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Gene Symbol

AGT

UniProt

ABM85122

Additional Serpin A8/Angiotensinogen Products

Product Documents for Human Serpin A8/Angiotensinogen Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin A8/Angiotensinogen Biotinylated Antibody

For research use only

Citations for Human Serpin A8/Angiotensinogen Biotinylated Antibody

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