Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin A8/Angiotensinogen Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen
Asp34-Ala485
Accession # ABM85122

Specificity

Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Serpin A8/Angiotensinogen is observed, and less than 5% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Serpin A8/Angiotensinogen Antibody

Detection of Human Serpin A8/Angiotensinogen antibody by Western Blot.

Detection of Human Serpin A8/Angiotensinogen by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A8/Angiotensinogen antibody in Human Brain by Immunohistochemistry (IHC-P).

Serpin A8/Angiotensinogen in Human Brain.

Serpin A8/Angiotensinogen was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Serpin A8/Angiotensinogen antibody by Simple WesternTM.

Detection of Human Serpin A8/Angiotensinogen by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin A8/Angiotensinogen at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Serpin A8/Angiotensinogen Antibody

Application
Recommended Usage

Immunohistochemistry

2-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human angiotensinogen is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.        

Alternate Names

AGT, Angiotensin II, Angiotensinogen, Aogen, PAT

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Gene Symbol

AGT

UniProt

ABM85122

Additional Serpin A8/Angiotensinogen Products

Product Documents for Human Serpin A8/Angiotensinogen Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin A8/Angiotensinogen Antibody

For research use only

Citations for Human Serpin A8/Angiotensinogen Antibody

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Protocols

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