Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC1455G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.
Entrez Gene IDs:
213 (Human); 11657 (Mouse); 24186 (Rat)
ALB; Albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin
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Citations: Showing 1 -
Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury
Authors: M Pyzik, T Rath, TT Kuo, S Win, K Baker, JJ Hubbard, R Grenha, A Gandhi, TD Krämer, AR Mezo, ZS Taylor, K McDonnell, V Nienaber, JT Andersen, A Mizoguchi, L Blumberg, S Purohit, SD Jones, G Christians, WI Lencer, I Sandlie, N Kaplowitz, DC Roopenian, RS Blumberg
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Sample Type: Whole Cells
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