Human Serum Albumin Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Human Albumin by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Albumin in Hepatocytes Derived from Human Embryonic Stem Cells. Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Albumin in Human Liver. Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Serum Albumin in Human Liver Using Dual RNAscope®ISH and IHC. Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope®Probe (Catalog # 600941) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.05 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Albumin
Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.
Product Datasheets
Citations for Human Serum Albumin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Oxygen Gradient Induced in Microfluidic Chips Can Be Used as a Model for Liver Zonation
Authors: S Ghafoory, C Stengl, S Kopany, M Mayadag, N Mechtel, B Murphy, S Schattschn, N Wilhelmi, S Wölfl
Cells, 2022;11(23):.
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF -
CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout
Authors: MAS de Toledo, X Fu, F Kluge, K Götz, S Schmitz, P Wanek, HM Schüler, K Pannen, N Chatain, S Koschmiede, TH Brümmendor, M Zenke
Stem Cell Research, 2022;60(0):102732.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients
Authors: M Ramirez-Ga, V Berge, A Lin?, A Llorente
British Journal of Cancer, 2021;0(0):.
Species: Human
Sample Types: Extracellular Vesicles
Applications: Western Blot -
Plasmodium vivax liver stage assay platforms using Indian clinical isolates
Authors: PA Subramani, N Vartak-Sha, S Sreekumar, P Mathur, B Nayer, S Dakhore, SK Basavanna, DM Kalappa, RV Krishnamur, B Mukhi, P Mishra, N Yoshida, SK Ghosh, R Shandil, S Narayanan, B Campo, K Hasegawa, AR Anvikar, N Valecha, V Sundaramur
Malar. J., 2020;19(1):214.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs
Authors: F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore
Cell Rep, 2020;33(9):108453.
Species: Human
Sample Types: Organoid
Applications: IHC -
Fecal host biomarkers predicting severity of Clostridioides difficile infection
Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
JCI Insight, 2020;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Repair of acute liver damage with immune evasive hESC derived hepato-blasts
Authors: J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li
Stem Cell Res, 2020;49(0):102010.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mechano-modulatory synthetic niches for liver organoid derivation
Authors: G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans
Nat Commun, 2020;11(1):3416.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
Authors: N Mori, Y Akagi, Y Imai, Y Takayama, YS Kida
Sci Rep, 2020;10(1):5646.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice
Authors: ES Famulari, V Navarro-Ta, MB Herrera Sa, G Bortolussi, M Gai, L Conti, L Silengo, E Tolosano, C Tetta, AF Muro, G Camussi, S Fagoonee, F Altruda
Sci Rep, 2020;10(1):887.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia
Authors: H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri
Sci Rep, 2019;9(1):4695.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
In vitro metabolic zonation through oxygen gradient on a chip
Authors: F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore
Sci Rep, 2019;9(1):13557.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
Authors: AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl
Sci Rep, 2019;9(1):10136.
Species: Human
Sample Types: Peritoneal Dialysate
Applications: ELISA Detection -
The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium
Authors: C Gu, P Li, W Liu, Y Zhou, WS Tan
J. Cell. Mol. Med., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
Authors: A Skrzypczyk, S Kehr, I Krystel, SH Bernhart, S Giri, A Bader, PF Stadler
Stem Cells Int, 2018;2018(0):5692840.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
4 in 1: Antibody-free protocol for isolating the main hepatic cells from healthy and cirrhotic single rat livers
Authors: A Fernández-, M Ortega-Rib, S Guixé-Munt, J Gracia-San
J. Cell. Mol. Med., 2018;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Nucleic acid-based polymers effective against hepatitis B Virus infection in patients don't harbor immunostimulatory properties in primary isolated liver cells
Authors: CI Real, M Werner, A Paul, G Gerken, JF Schlaak, A Vaillant, R Broering
Sci Rep, 2017;7(0):43838.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
Authors: MB Herrera Sa, S Previdi, S Bruno, V Fonsato, MC Deregibus, S Kholia, S Petrillo, E Tolosano, R Critelli, M Spada, R Romagnoli, M Salizzoni, C Tetta, G Camussi
Stem Cell Res Ther, 2017;8(1):176.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
Nat Commun, 2016;7(0):13363.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model
Stem Cells Dev, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr -
Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes.
Authors: Park H, Choi Y, Kim J, Chun H, Im I, Yoon S, Han Y, Song C, Kim H
PLoS ONE, 2015;10(7):e0132992.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fatty acid-binding proteins (FABPs) are intracellular carriers for Delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD).
Authors: Elmes M, Kaczocha M, Berger W, Leung K, Ralph B, Wang L, Sweeney J, Miyauchi J, Tsirka S, Ojima I, Deutsch D
J Biol Chem, 2015;290(14):8711-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Production of hepatocyte-like cells from human pluripotent stem cells.
Authors: Hannan, Nicholas, Segeritz, Charis-P, Touboul, Thomas, Vallier, Ludovic
Nat Protoc, 2013;8(2):430-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Identification of transcription factors for lineage-specific ESC differentiation.
Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
Stem Cell Reports, 2013;1(6):545-59.
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Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
PLoS ONE, 2012;7(5):e37917.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Authors: Hoang S, Liauw J, Choi M
J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Fate-mapping evidence that hepatic stellate cells are epithelial progenitors in adult mouse livers.
Authors: Yang L, Jung Y, Omenetti A, Witek RP, Choi S, Vandongen HM, Huang J, Alpini GD, Diehl AM
Stem Cells, 2008;26(8):2104-13.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Stem cells derived from human fetal membranes display multilineage differentiation potential.
Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
Biol. Reprod., 2007;77(3):577-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
J. Biol. Chem., 2007;282(32):23337-47.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Isolation and characterization of a stem cell population from adult human liver.
Authors: Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G
Stem Cells, 2006;24(12):2840-50.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro.
Authors: Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A
Int. J. Dev. Biol., 2006;50(7):645-52.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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