Human Siglec-10 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human Siglec-10 Biotinylated Antibody Summary

Species Reactivity
Detects human Siglec-10 in Western blots. In Western blots, less than 2% cross-reactivity with recombiant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Accession # Q96LC7
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Siglec‑10 Fc Chimera (Catalog # 2130-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

  1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
  7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
  8. Munday, J. et al. (2001) Biochem. J. 355:489.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
  10. Aizawa, H. et al. (2003) Genomics 82:521.
  11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
Long Name
Sialic Acid Binding Ig-like Lectin 10
Entrez Gene IDs
89790 (Human)
Alternate Names
MGC126774; PRO940; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; Siglec10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

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Citation for Human Siglec-10 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction.
    Authors: Chen GY, Chen X, King S
    Nat. Biotechnol., 2011;29(5):428-35.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Flow Cytometry


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