Human SIRP alpha/CD172a Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4546N-100UG
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Human SIRP alpha/CD172a Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SIRP alpha /CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta 1 and no cross-reactivity with rhSIRP beta 2 is observed.
Source
Monoclonal Mouse IgG2b Clone # 602411
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha /CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins.  It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts.  SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis.  It binds to CD47 and likely other ligands.  Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein.  It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347).  Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases.  There is one alternative start site at Met102 plus a four aa insertion after Gln421.  Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.

Long Name
Signal-regulatory Protein alpha
Entrez Gene IDs
140885 (Human); 19261 (Mouse); 25528 (Rat); 101926317 (Cynomolgus Monkey)
Alternate Names
BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1

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