Human SIRP alpha /CD172a
Accession # P78324
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SIRP alpha /CD172a by Western Blot. Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SIRPa/CD172a at approximately 70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SIRP alpha /CD172a
by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP alpha /CD172a
Monoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of SIRP alpha /CD172a in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
SIRP alpha /CD172a in THP‑1 Human Cell Line. SIRPa/CD172a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Citation for Human SIRP alpha /CD172a
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
TRIM2, a novel member of the antiviral family, limits New World arenavirus entry
Authors: N Sarute, N Ibrahim, B Medegan Fa, M Lavanya, C Cuevas, S Stavrou, G Otkiran-Cl, H Tyynismaa, J Henao-Meji, SR Ross
PLoS Biol., 2019;17(2):e3000137.
Sample Types: Whole Cells
Applications: Flow Cytometry
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