SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Human SIRP alpha /CD172a
Antibody
R&D Systems | Catalog # MAB4546
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Gly27-Asn370 (predicted)
Accession # P78324
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human SIRP alpha /CD172a
Antibody
Detection of Human SIRP alpha /CD172a
by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP alpha /CD172a
Monoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SIRP alpha /CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of SIRP alpha /CD172a in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
SIRP alpha /CD172a in THP‑1 Human Cell Line.
SIRPa/CD172a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Applications for Human SIRP alpha /CD172a Antibody
CyTOF-ready
Flow Cytometry
Sample: U937 human histiocytic lymphoma cell line
Immunocytochemistry
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Western Blot
Sample: A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes
Reviewed Applications
Read 3 reviews rated 4.3 using MAB4546 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SIRP alpha/CD172a
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional SIRP alpha/CD172a Products
Product Documents for Human SIRP alpha /CD172a Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SIRP alpha /CD172a Antibody
For research use only
Citations for Human SIRP alpha /CD172a
Antibody
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Customer Images
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: fibroblastsSpecies: HumanVerified Customer | Posted 11/10/2021
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Melanoma tissueSpecies: HumanVerified Customer | Posted 11/23/2020
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Application: Western BlotSample Tested: MACROPHAGESpecies: HumanVerified Customer | Posted 04/29/2020
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
