Human SLPI Antibody

(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with mouse SLPI is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SLPI
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human SLPI (Catalog # 1274-PI)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Recombinant Human SLPI (0.125 µg/mL, Catalog # 1274-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 2 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
SLPI in Human Lung.

SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLPI
SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).
  • References:
    1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
    2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.
  • Long Name:
    Secretory Leukocyte Protease Inhibitor
  • Entrez Gene IDs:
    6590 (Human); 20568 (Mouse)
  • Alternate Names:
    ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-beta signaling protein Smad2.
    Authors: Hannila S, Siddiq M, Carmel J, Hou J, Chaudhry N, Bradley P, Hilaire M, Richman E, Hart R, Filbin M
    J Neurosci, 2013;33(12):5138-51.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  2. Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma.
    Authors: Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM
    Am. J. Pathol., 2011;178(6):2866-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelins.
    Authors: Edstrom AM, Malm J, Frohm B, Martellini JA, Giwercman A, Morgelin M, Cole AM, Sorensen OE
    J. Immunol., 2008;181(5):3413-21.
    Species: Human
    Sample Type: Seminal Plasma
    Application: WB
  4. The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils.
    Authors: Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K
    J. Leukoc. Biol., 2008;83(5):1155-64.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  5. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008;7(10):1974-82.
    Species: Human
    Sample Type: Serum
    Application: MS
  6. The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells.
    Authors: Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC
    Carcinogenesis, 2007;29(3):466-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model.
    Authors: Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E
    Lab. Invest., 2004;84(3):320-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Antimicrobial components of vaginal fluid.
    Authors: Valore EV, Park CH, Igreti SL, Ganz T
    Am. J. Obstet. Gynecol., 2002;187(3):561-8.
    Species: Human
    Sample Type: Vaginal Fluid
    Application: WB
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