Human SLPI Antibody

Catalog # Availability Size / Price Qty
AF1274
AF1274-SP
SLPI in Human Lung.
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Product Details
Citations (9)
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Human SLPI Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SLPI
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SLPI (Catalog # 1274-PI)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Human SLPI (0.125 µg/mL, Catalog # 1274-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 2 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry SLPI in Human Lung. View Larger

SLPI in Human Lung.

SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References
  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.
Long Name
Secretory Leukocyte Protease Inhibitor
Entrez Gene IDs
6590 (Human); 20568 (Mouse)
Alternate Names
ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI

Product Datasheets

Citations for Human SLPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity
    Authors: QH Nguyen, N Pervolarak, K Blake, D Ma, RT Davis, N James, AT Phung, E Willey, R Kumar, E Jabart, I Driver, J Rock, A Goga, SA Khan, DA Lawson, Z Werb, K Kessenbroc
    Nat Commun, 2018;9(1):2028.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-beta signaling protein Smad2.
    Authors: Hannila S, Siddiq M, Carmel J, Hou J, Chaudhry N, Bradley P, Hilaire M, Richman E, Hart R, Filbin M
    J Neurosci, 2013;33(12):5138-51.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinoma.
    Authors: Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, Wahl SM
    Am. J. Pathol., 2011;178(6):2866-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008;7(10):1974-82.
    Species: Human
    Sample Types: Serum
    Applications: Mass Spectrometry
  5. The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils.
    Authors: Jacobsen LC, Sorensen OE, Cowland JB, Borregaard N, Theilgaard-Monch K
    J. Leukoc. Biol., 2008;83(5):1155-64.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelins.
    Authors: Edstrom AM, Malm J, Frohm B, Martellini JA, Giwercman A, Morgelin M, Cole AM, Sorensen OE
    J. Immunol., 2008;181(5):3413-21.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Western Blot
  7. The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells.
    Authors: Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC
    Carcinogenesis, 2007;29(3):466-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model.
    Authors: Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E
    Lab. Invest., 2004;84(3):320-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Antimicrobial components of vaginal fluid.
    Authors: Valore EV, Park CH, Igreti SL, Ganz T
    Am. J. Obstet. Gynecol., 2002;187(3):561-8.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: Western Blot

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