Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Bacteria - Borrelia burgdorferi, Bacteria - Borrelia burgdorferi (Lyme disease spirochete), Bacteria - Stenotrophomonas maltophilia

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), ELISA Detection, Mass Spectrometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SLPI Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SLPI

Specificity

Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SLPI Antibody

SLPI antibody in Human Lung by Immunohistochemistry (IHC-P).

SLPI in Human Lung.

SLPI was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1274) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SLPI Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Western Blot

0.1 µg/mL
Sample: Recombinant Human SLPI (Catalog # 1274-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human SLPI (0.125 µg/mL, Catalog # 1274-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 2 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References

  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.

Long Name

Secretory Leukocyte Protease Inhibitor

Alternate Names

ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI

Entrez Gene IDs

6590 (Human); 20568 (Mouse)

Gene Symbol

SLPI

Additional SLPI Products

Product Documents for Human SLPI Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SLPI Antibody

For research use only

Citations for Human SLPI Antibody

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Protocols

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