Human Smoothened Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB10276N Datasheet / COA / SDS

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FAB10276N-100UG

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Human Smoothened Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Smoothened in direct ELISAs.

Source

Monoclonal Mouse IgG2a Clone # 1015418

Purification

Protein A or G purified

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Smoothened
Met1-Tyr233
Accession # Q99835

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Smoothened

Smoothened homolog (SMO) is a G protein-coupled receptor that is a component of the hedgehog signaling pathway. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and serve as driving mutations for cancers such as medulloblastoma, basal-cell carcinoma, pancreatic cancer, and prostate cancer. As such, SMO is an attractive cancer drug target, along with the many hedgehog pathway agonists and antagonists that are known to directly target SMO.

Entrez Gene IDs

6608 (Human); 319757 (Mouse); 25273 (Rat)

Alternate Names

bnb; CRJS; FZD11; Gx; Protein Gx; SMO; SMOH; SMOHseven transmembrane helix receptor; smoothened (Drosophila) homolog; smoothened homolog (Drosophila); smoothened homolog; Smoothened