Human Smoothened Antibody

Catalog # Availability Size / Price Qty
MAB10276-100
MAB10276-SP
Human Smoothened Antibody in Immunohistochemistry
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Human Smoothened Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smoothened in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1015418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Smoothened
Met1-Tyr233
Accession # Q99835
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Smoothened in Human Liver Cancer Tissue. View Larger

Smoothened in Human Liver Cancer Tissue. Smoothened was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Smoothened Monoclonal Antibody (Catalog # MAB10276) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smoothened

Smoothened homolog (SMO) is a G protein-coupled receptor that is a component of the hedgehog signaling pathway. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and serve as driving mutations for cancers such as medulloblastoma, basal-cell carcinoma, pancreatic cancer, and prostate cancer. As such, SMO is an attractive cancer drug target, along with the many hedgehog pathway agonists and antagonists that are known to directly target SMO.

Entrez Gene IDs
6608 (Human); 319757 (Mouse); 25273 (Rat)
Alternate Names
bnb; CRJS; FZD11; Gx; Protein Gx; SMO; SMOH; SMOHseven transmembrane helix receptor; smoothened (Drosophila) homolog; smoothened homolog (Drosophila); smoothened homolog; Smoothened

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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