Detection of Snail in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3639G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.
Have you used Human Snail Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.