Human Snail Antibody

Catalog # Availability Size / Price Qty
AF3639
AF3639-SP
Detection of Snail in A549 Human Cell Line by Flow Cytometry.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (4)

Human Snail Antibody Summary

Species Reactivity
Human
Specificity
Detects human Snail in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Snail
Pro2-Arg264
Accession # O95863
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of Snail antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Snail in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Chromatin Immunoprecipitation (ChIP) Detection of Snail-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of Snail-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheE-Cadherinpromoter was detected by standard PCR.

Western Blot Detection of Human Snail antibody by Western Blot. View Larger

Detection of Human Snail by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Snail at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Snail antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

Snail in A549 Human Cell Line. Snail was detected in immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (left panel, Catalog # 240-B) or untreated (right panel) using Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Snail

Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.

Entrez Gene IDs
6615 (Human); 20613 (Mouse); 116490 (Rat)
Alternate Names
dJ710H13.1; Protein sna; Protein snail homolog 1; SLUGH2; SNA; SNAH; SNAI1; snail 1 homolog; snail 1 zinc finger protein; snail family zinc finger 1; snail homolog 1; Snail; SNAIL1; zinc finger protein SNAI1

Product Datasheets

Citations for Human Snail Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide
    Authors: NC Boles, M Fernandes, T Swigut, R Srinivasan, L Schiff, A Rada-Igles, Q Wang, JS Saini, T Kiehl, JH Stern, J Wysocka, TA Blenkinsop, S Temple
    Stem Cell Reports, 2020;14(4):631-647.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma
    Authors: M Kurihara-S, T Sasahira, H Shimomura, C Nakashima, T Kirita
    J Clin Med, 2019;8(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases
    Authors: JM Oh, J Lee, WT Im, S Chun
    Int J Mol Sci, 2019;20(5):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21
    Authors: K Fukuura, Y Inoue, C Miyajima, S Watanabe, M Tokugawa, D Morishita, N Ohoka, M Komada, H Hayashi
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression
    Authors: M Rokavec, M Kaller, D Horst, H Hermeking
    Sci Rep, 2017;7(1):4687.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  6. Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGF? stimulation in cancer.
    Authors: Shyam Kumar Gudey, Reshma Sundar, Carl-Henrik Heldin, Anders Bergh, Marene Landström
    Oncotarget, 2017;0(0):1949-2553.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
    Authors: Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang
    British Journal of Cancer, 2017;0(0):1532-1827.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  8. Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
    Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
    Clin Cancer Res, 2015;21(4):899-906.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. SNAI1 expression and the mesenchymal phenotype: an immunohistochemical study performed on 46 cases of oral squamous cell carcinoma.
    Authors: Schwock J, Bradley G, Ho JC
    BMC Clin Pathol, 2010;10(0):1.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human Snail Antibody

Average Rating: 3.8 (Based on 4 Reviews)

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Human Snail Antibody
By Anonymous on 10/17/2019
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human

Human Snail Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: HCT-116 human colorectal carcinoma cell line,HeLa human cervical epithelial carcinoma cell line,PC-3 human prostate cancer cell line Species: Human

Total cell lysates from HCT-116, HeLa and PC-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human Snail Antibody (AF3639). A specific band was detected for Snail at approximately 30 kDa. This experiment was conducted under reducing conditions.


Human Snail Antibody
By Anonymous on 12/10/2017
Application: ICC/IF Sample Tested: Bladder cancer tissue Species: Human

Human Snail Antibody
By Anonymous on 10/18/2016
Application: ICC/IF Sample Tested: mesenchymal cells Species: Human