Human Snail Antibody
Human Snail Antibody Summary
Pro2-Arg264
Accession # O95863
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Snail by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Snail at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Snail-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheE-Cadherinpromoter was detected by standard PCR.
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Snail in A549 Human Cell Line. Snail was detected in immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (left panel, Catalog # 240-B) or untreated (right panel) using Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Snail in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Snail
Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.
Product Datasheets
Citations for Human Snail Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer
Authors: JD Twomey, B Zhang
Biomedicines, 2023;11(3):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells
Authors: M Simunovic, ED Siggia, AH Brivanlou
Cell Stem Cell, 2022;29(6):962-972.e4.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide
Authors: NC Boles, M Fernandes, T Swigut, R Srinivasan, L Schiff, A Rada-Igles, Q Wang, JS Saini, T Kiehl, JH Stern, J Wysocka, TA Blenkinsop, S Temple
Stem Cell Reports, 2020;14(4):631-647.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/beta-catenin pathway
Authors: R Zhang, J Liu, W Zhang, L Hua, LT Qian, SB Zhou
Cancer Cell Int., 2020;20(0):20.
Species: Human
Sample Types: Cell Lysates
Applications: IF -
The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma
Authors: M Kurihara-S, T Sasahira, H Shimomura, C Nakashima, T Kirita
J Clin Med, 2019;8(2):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases
Authors: JM Oh, J Lee, WT Im, S Chun
Int J Mol Sci, 2019;20(5):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21
Authors: K Fukuura, Y Inoue, C Miyajima, S Watanabe, M Tokugawa, D Morishita, N Ohoka, M Komada, H Hayashi
J. Biol. Chem., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Myoferlin Contributes to the Metastatic Phenotype of Pancreatic Cancer Cells by Enhancing Their Migratory Capacity through the Control of Oxidative Phosphorylation
Authors: G Rademaker, B Costanza, S Anania, F Agirman, N Maloujahmo, E Di Valenti, JJ Goval, A Bellahcène, V Castronovo, O Peulen
Cancers (Basel), 2019;11(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression
Authors: M Rokavec, M Kaller, D Horst, H Hermeking
Sci Rep, 2017;7(1):4687.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP -
Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGF? stimulation in cancer.
Authors: Shyam Kumar Gudey, Reshma Sundar, Carl-Henrik Heldin, Anders Bergh, Marene Landström
Oncotarget, 2017;0(0):1949-2553.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells.
Authors: Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang
British Journal of Cancer, 2017;0(0):1532-1827.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
Clin Cancer Res, 2015;21(4):899-906.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
SNAI1 expression and the mesenchymal phenotype: an immunohistochemical study performed on 46 cases of oral squamous cell carcinoma.
Authors: Schwock J, Bradley G, Ho JC
BMC Clin Pathol, 2010;10(0):1.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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Reviews for Human Snail Antibody
Average Rating: 3.8 (Based on 4 Reviews)
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Total cell lysates from HCT-116, HeLa and PC-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human Snail Antibody (AF3639). A specific band was detected for Snail at approximately 30 kDa. This experiment was conducted under reducing conditions.
