Human SOST/Sclerostin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM14061
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Human SOST/Sclerostin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOST/Sclerostin in ELISAs. In sandwich immunoassays, 30% cross-reactivity with recombinant mouse SOST is observed and no cross-reactivity or interference with recombinant human BMP-2, -4, -5, -6, or -7 was observed.
Source
Monoclonal Mouse IgG1 Clone # 220910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SOST/Sclerostin
Gln24-Tyr213
Accession # Q9BQB4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human SOST Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human SOST/Sclerostin Antibody (Catalog # MAB1406)

Standard: Recombinant Human SOST/Sclerostin Protein (Catalog # 1406-ST)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1‑3).

References
  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Entrez Gene IDs
50964 (Human); 74499 (Mouse)
Alternate Names
sclerostin; SOST; VBCHsclerosteosis

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