Human SPARC Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DSP00
Control Products Available
Human SPARC ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human SPARC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor EDTA Plasma (20 uL), Platelet-poor Heparin Plasma (20 uL), Human Milk (50 uL)
Sensitivity
0.269 ng/mL
Assay Range
1.6 - 50 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Human Milk)
Specificity
Natural and recombinant human SPARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SPARC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SPARC in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human SPARC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SPARC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SPARC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 7.34 14.3 29.9 6.65 13.5 28.3
Standard Deviation 0.129 0.282 0.733 0.541 0.989 2.4
CV% 1.8 2 2.5 8.1 7.3 8.5

Recovery

The recovery of SPARC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 86-111
Platelet-poor EDTA Plasma (n=4) 104 93-111
Platelet-poor Heparin Plasma (n=4) 91 82-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SPARC were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human SPARC ELISA Linearity

Data Examples

Human SPARC ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SPARC

SPARC, also known as Osteonectin and Basement Membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage, and morphogenic processes such as re-modeling of the extracellular matrix.

Long Name:
Secreted Protein Acidic and Rich in Cysteine
Entrez Gene IDs:
6678 (Human); 20692 (Mouse)
Alternate Names:
Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour on the benchtop.
  10.   Aspirate and wash 6 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human SPARC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Impact of Fibroblast-Derived SPARC on Invasiveness of Colorectal Cancer Cells
    Authors: D Drev, F Harpain, A Beer, A Stift, ES Gruber, M Klimpfinge, S Thalhammer, A Reti, L Kenner, M Bergmann, B Marian
    Cancers (Basel), 2019;11(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Lowered Decorin With Aberrant Extracellular Matrix Remodeling in Aqueous Humor and Tenon's Tissue From Primary Glaucoma Patients
    Authors: S NikhalaShr, G Karthikkey, R George, B Shantha, L Vijaya, V Ratra, KN Sulochana, K Coral
    Invest. Ophthalmol. Vis. Sci., 2019;60(14):4661-4669.
    Species: Human
    Sample Types: Aqueous Humor
  3. Secreted protein acidic and rich in cysteine mediates active targeting of human serum albumin in U87MG xenograft mouse models
    Authors: CR Park, JH Jo, MG Song, JY Park, YH Kim, H Youn, SH Paek, JK Chung, JM Jeong, YS Lee, KW Kang
    Theranostics, 2019;9(24):7447-7457.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. SPARC expression is associated with hepatic injury in rodents and humans with non-alcoholic fatty liver disease
    Authors: G Mazzolini, C Atorrasaga, A Onorato, E Peixoto, M Schlattjan, JP Sowa, S Sydor, G Gerken, A Canbay
    Sci Rep, 2018;8(1):725.
    Species: Human
    Sample Types: Serum
  5. In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery
    Authors: J Zhang, N Hao, W Liu, M Lu, L Sun, N Chen, M Wu, X Zhao, B Xing, W Sun, F He
    Br. J. Cancer, 2017;117(11):1676-1684.
    Species: Human
    Sample Types: Serum
  6. SPARC Expression Did Not Predict Efficacy of nab-Paclitaxel plus Gemcitabine or Gemcitabine Alone for Metastatic Pancreatic Cancer in an Exploratory Analysis of the Phase III MPACT Trial.
    Authors: Hidalgo M, Plaza C, Musteanu M, Illei P, Brachmann C, Heise C, Pierce D, Lopez-Casas P, Menendez C, Tabernero J, Romano A, Wei X, Lopez-Rios F, Von Hoff D
    Clin Cancer Res, 2015;21(21):4811-8.
    Species: Human
    Sample Types: Plasma
  7. SPARC expression in CML is associated to imatinib treatment and to inhibition of leukemia cell proliferation.
    Authors: Giallongo C, La Cava P, Tibullo D, Barbagallo I, Parrinello N, Cupri A, Stagno F, Consoli C, Chiarenza A, Palumbo G, Di Raimondo F
    BMC Cancer, 2013;13(0):60.
    Species: Human
    Sample Types: Serum

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

ELISA Controls

Supplemental ELISA Products

Reviews for Human SPARC Quantikine ELISA Kit

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human SPARC Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human SPARC Quantikine ELISA Kit
By Anonymous on 11/21/2018
Application: Sample Tested: Cell culture supernatant,Tissue Culture Media