Human SSBP1 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SSBP1

SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.

Product Datasheets

Related Research Areas

• DNA Damage and Repair