Human SSBP1 Antibody

Catalog # Availability Size / Price Qty
AF6588
AF6588-SP
Human SSBP1 Antibody in Western Blot
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human SSBP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SSBP1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SSBP1
Met1-Glu148
Accession # Q04837
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human SSBP1 by Western Blot. View Larger

Detection of Human SSBP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and UT-7 human acute myeloid leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SSBP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry SSBP1 in HL‑60 Human Cell Line. View Larger

SSBP1 in HL‑60 Human Cell Line. SSBP1 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSBP1

SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.

Long Name
Single-stranded DNA Binding Protein Motochondrial
Entrez Gene IDs
6742 (Human); 381760 (Mouse); 54304 (Rat)
Alternate Names
mitochondrial; MtSSB; single-stranded DNA binding protein 1; SSBP1

Product Datasheets

Citation for Human SSBP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
    Authors: C Piro-Mégy, E Sarzi, A Tarrés-Sol, M Péquignot, F Hensen, M Quilès, G Manes, A Chakrabort, A Sénéchal, B Bocquet, C Cazevieill, A Roubertie, A Müller, M Charif, D Goudenège, G Lenaers, H Wilhelm, U Kellner, N Weisschuh, B Wissinger, X Zanlonghi, C Hamel, JN Spelbrink, M Solà, C Delettre
    J. Clin. Invest., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human SSBP1 Antibody

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Human SSBP1 Antibody
By Anonymous on 11/14/2017
Application: WB Sample Tested: H1299 human lung carcinoma cell line,H1944 human lung carcinoma cell line,Calu-6 human lung carcinoma cell line Species: Human