Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SSBP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SSBP1
Met1-Glu148
Accession # Q04837

Specificity

Detects human SSBP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SSBP1 Antibody

Detection of Human SSBP1 antibody by Western Blot.

Detection of Human SSBP1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and UT-7 human acute myeloid leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SSBP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SSBP1 antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

SSBP1 in HL‑60 Human Cell Line.

SSBP1 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human SSBP1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HL-60 human acute promyelocytic leukemia cell line

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and UT‑7 human acute myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 4 using AF6588 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SSBP1

SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.

Long Name

Single-stranded DNA Binding Protein Motochondrial

Alternate Names

mitochondrial, MtSSB, single-stranded DNA binding protein 1

Entrez Gene IDs

6742 (Human); 381760 (Mouse); 54304 (Rat)

Gene Symbol

SSBP1

UniProt

Q04837

Additional SSBP1 Products

Product Documents for Human SSBP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SSBP1 Antibody

For research use only

Citations for Human SSBP1 Antibody

Customer Reviews for Human SSBP1 Antibody (1)

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  • Human SSBP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: H1299 human lung carcinoma cell line, H1944 human lung carcinoma cell line and Calu-6 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/14/2017
    Human SSBP1 Antibody AF6588

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