Human ST8 alpha -2,8‑Sialyltransferase 8B/
ST8SIA2 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF6590AFP405

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Sheep IgG
View all ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Primary Antibodies »

Product Specifications

Specificity

Detects human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2

ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).

References

  1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
  2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
  3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
  4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
  5. Ong, E. et al. (1998). Glycobiology 8:415.
  6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
  7. Thompson, M. G. et al. (2010) J. Biol. Chem. in press.
  8. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2

Alternate Names

SIAT8B, ST8SiaII, STX

Entrez Gene IDs

8128 (Human)

Gene Symbol

ST8SIA2

UniProt

Q92186

Additional ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Glycobiology-related Enzymes

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