Human ST8 alpha -2,8‑Sialyltransferase 8B/
ST8SIA2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human alpha -2,8‑Sialyltransferase 8B/ST8SIA2
    Asp24-Thr375
    Accession # Q92186
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line, Saos‑2 human osteosarcoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human alpha -2,8‑Sialyltransferase 8B/
ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha -2,8‑Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
alpha -2,8‑Sialyltransferase 8B/ ST8SIA2 in Mouse Embryo. alpha ‑2,8‑Sialyltransferase 8B/ ST8SIA2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human alpha ‑2,8‑Sialyltransferase 8B/
ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of alpha ‑2,8‑Sialyltransferase 8B/ST8SIA2 in IMR‑32 Human Cell Line by Flow Cytometry.

IMR‑32 human neuroblastoma cell line was stained with Sheep Anti-Human alpha -2,8‑Sialyltransferase 8B/
ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

 

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2
ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).
  • References:
    1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
    2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
    3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
    4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
    5. Ong, E. et al. (1998). Glycobiology 8:415.
    6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
    7. Thompson, M. G. et al. (2010) J. Biol. Chem. in press.
    8. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.
  • Long Name:
    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
  • Entrez Gene IDs:
    8128 (Human)
  • Alternate Names:
    alpha-2,8-sialyltransferase 8B 1; alpha-2,8-sialyltransferase 8B; EC 2.4.99; EC 2.4.99.-; HsT19690; MGC116854; MGC116857; sialyltransferase 8 (alpha-2, 8-sialytransferase) B; Sialyltransferase 8B; Sialyltransferase X; Sialytransferase St8Sia II; SIAT8B; SIAT8-B; ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2; ST8SIA2; ST8SiaII; ST8SIA-II; STX; STXST8SiaII
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