Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

R&D Systems | Catalog # AF6590

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human alpha -2,8‑Sialyltransferase 8B/ST8SIA2
Asp24-Thr375
Accession # Q92186

Specificity

Detects human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Detection of Human a-2,8-Sialyltransferase 8B/ST8SIA2 antibody by Western Blot.

Detection of Human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 by Western Blot.

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, Saos-2 human osteosarcoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-2,8-Sialyltransferase 8B/ ST8SIA2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

alpha -2,8‑Sialyltransferase 8B/ ST8SIA2 in Mouse Embryo.

a-2,8-Sialyltransferase 8B/ ST8SIA2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of a-2,8-Sialyltransferase 8B/ST8SIA2 antibody in IMR-32 Human Cell Line antibody by Flow Cytometry.

Detection of alpha ‑2,8‑Sialyltransferase 8B/ST8SIA2 in IMR‑32 Human Cell Line by Flow Cytometry.

IMR-32 human neuroblastoma cell line was stained with Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: IMR‑32 human neuroblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.2 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line, Saos‑2 human osteosarcoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2

ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).

References

  1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
  2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
  3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
  4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
  5. Ong, E. et al. (1998). Glycobiology 8:415.
  6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
  7. Thompson, M. G. et al. (2010) J. Biol. Chem. in press.
  8. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2

Alternate Names

SIAT8B, ST8SiaII, STX

Entrez Gene IDs

8128 (Human)

Gene Symbol

ST8SIA2

UniProt

Q92186

Additional ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Products

Product Documents for Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Certificate of Analysis

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Product Specific Notices for Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Human ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

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Protocols

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